A Near-infrared Light-mediated Cleavable Linker Strategy Using the Heptamethine Cyanine Chromophore

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2020 Jul 28

PMID 32713525

Citations 7

Authors

Michael P Luciano

Saghar Nourian

Alexander P Gorka

Roger R Nani

Tadanobu Nagaya

Hisataka Kobayashi

Martin J Schnermann

Affiliations

Soon will be listed here.

Abstract

Optical methods offer the potential to manipulate living biological systems with exceptional spatial and temporal control. Caging bioactive molecules with photocleavable functional groups is an important strategy that could be applied to a range of problems, including the targeted delivery of otherwise toxic therapeutics. However existing approaches that require UV or blue light are difficult to apply in organismal settings due to issues of tissue penetration and light toxicity. Photocaging groups built on the heptamethine cyanine scaffold enable the targeted delivery of bioactive molecules using near-IR light (up to 780nm) in live animal settings. Here we provide a detailed procedure demonstrating the utility of the heptamethine cyanine caging group to create a light-cleavable linker between an antibody, panitumumab, and a therapeutic small molecule in the duocarmycin class of natural products. Descriptions of the design and synthesis of the small molecule component, assembly of the antibody conjugate, in vitro analysis of uncaging, in vivo imaging, and impact on tumor progression are provided.

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