Use of the Avidin-biotin Complex for the Localization of Actin and Myosin with Fluorescence Microscopy

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1977 Jun 1

PMID 326797

Citations 40

Authors

M H Heggeness

J F Ash

Affiliations

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Abstract

A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.

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