Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Jul 18

PMID 32674311

Citations 30

Authors

Elena Naumovska

Germaine Aalderink

Christian Wong Valencia

Kinga Kosim

Arnaud Nicolas

Stephen Brown

Paul Vulto

Kai S Erdmann

Dorota Kurek

Affiliations

Soon will be listed here.

Abstract

Intestinal organoids have emerged as the new paradigm for modelling the healthy and diseased intestine with patient-relevant properties. In this study, we show directed differentiation of induced pluripotent stem cells towards intestinal-like phenotype within a microfluidic device. iPSCs are cultured against a gel in microfluidic chips of the OrganoPlate, in which they undergo stepwise differentiation. Cells form a tubular structure, lose their stem cell markers and start expressing mature intestinal markers, including markers for Paneth cells, enterocytes and neuroendocrine cells. Tubes develop barrier properties as confirmed by transepithelial electrical resistance (TEER). Lastly, we show that tubules respond to pro-inflammatory cytokine triggers. The whole procedure for differentiation lasts 14 days, making it an efficient process to make patient-specific organoid tubules. We anticipate the usage of the platform for disease modelling and drug candidate screening.

Citing Articles

The Use of Gut Organoids: To Study the Physiology and Disease of the Gut Microbiota.

Deng Y, Yuan X, Lu X, Wu J, Luo C, Zhang T J Cell Mol Med. 2025; 29(4):e70330.

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Sun L, Chen H, Xu D, Liu R, Zhao Y Smart Med. 2024; 3(2):e20240009.

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