Intracellular Redox Perturbation in Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production

Overview

Journal Front Bioeng Biotechnol

Date 2020 Jul 14

PMID 32656198

Citations 10

Authors

Chen-Guang Liu

Kai Li

Ke-Yi Li

Chularat Sakdaronnarong

Muhammad Aamer Mehmood

Xin-Qing Zhao

Feng-Wu Bai

Affiliations

Soon will be listed here.

Abstract

Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of . The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on furfural tolerance. Here, three strategies were employed in cofactor conversion in : (1) heterologous expression of NADH dehydrogenase () from which catalyzed the NADH to NAD and increased the cellular sensitivity to furfural, (2) overexpression of , and genes responsible for the interconversion of NADPH and NADP, which enhanced the furfural tolerance, (3) expression of NAD(P) transhydrogenase () and NAD kinase () which showed a little impact on furfural tolerance. Besides, a substantial redistribution of metabolic fluxes was also observed with the expression of cofactor-related genes. These results indicated that NADPH-based intracellular redox perturbation plays a key role in furfural tolerance, which suggested single-gene manipulation as an effective strategy for enhancing tolerance and subsequently achieving higher ethanol titer using lignocellulosic hydrolysate.

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