The Genome Insights of Streptomyces Lannensis T1317-0309 Reveals Actinomycin D Production

Overview

Journal J Antibiot (Tokyo)

Specialties Infectious Diseases
Pharmacology

Date 2020 Jul 10

PMID 32641781

Citations 1

Authors

Ram Hari Dahal

Tuan Manh Nguyen

Ramesh Prasad Pandey

Tokutaro Yamaguchi

Jae Kyung Sohng

Jongsung Noh

Seung-Woon Myung

Jaisoo Kim

Affiliations

Soon will be listed here.

Abstract

The members of Streptomyces have been identified as a major source of antimicrobial agents with broad spectrum. This study is mainly focused on bioactivity-guided isolation and characterization of bioactive molecule from strain Streptomyces sp. T1317-0309 and its whole-genome sequence analysis for possible isolation of novel natural products. Strain Streptomyces sp. T1317-0309 showed 100% sequence similarity with strain Streptomyces lannensis TA4-8 consisting 10, 453,255 bp of genome with 5 scaffolds and 69.9 mol% G + C content. The genome analyses revealed a total of 17 putative biosynthetic gene clusters (BGCs) responsible for various secondary metabolites including actinomycin, bacteriocin, ectoine, melanin, terpene, siderophore, betalactone, NRPS, T2PKS, and T3PKS. The BGC and bioactivity-guided purification of ethyl acetate extract of strain T1317-0309 showed the great potency of antimicrobial activities against various gram-positive multi-drug resistant human pathogens including MRSA. The BGC-predicted bioactive secondary metabolite was identified by various NMR analyses and confirmed as actinomycin D. In addition, this study reveals the first genome study of Streptomyces lannensis as a novel source for actinomycin D.

Citing Articles

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Ma Y, Guo P, Chen X, Xu M, Liu W, Jin X BMC Microbiol. 2023; 23(1):162.

PMID: 37277707 PMC: 10240788. DOI: 10.1186/s12866-023-02909-7.

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