Frequency of the Rs 14035 Polymorphism of Gen in Recurrent Pregnancy Loss: A Case-control Study

Overview

Journal Int J Reprod Biomed

Specialty Reproductive Medicine

Date 2020 Jul 9

PMID 32637864

Authors

Zahrasadat Mortazavifar

Hamidreza Ashrafzadeh

Seyed Morteza Seifati

Nasrin Ghasemi

Affiliations

Soon will be listed here.

Abstract

Background: Genetic factors could account for recurrent pregnancy loss (RPL). The gene is a member of the "large RAS family" and a small GTPase that is essential for the translocation of Ribonucleic acid (RNA) and proteins through the nuclear pore. Mutation in the constitutive gene could stop DNA synthesis and alter the expression of genes in the uterus, likely playing a role in recurrent miscarriage.

Objective: The aim was to investigate the frequency of (rs 14035) polymorphism in women with RPL compared with women without abortion history.

Materials And Methods: In this case-control study, 100 women with at least two consecutive miscarriages before the 20th wk of gestation and having spouses with karyotype and normal sperm parameters as the case group and 100 women with no history of abortion and having at least one successful pregnancy and normal delivery as the control group. The groups were age matched (20-40 yr). The rs 14035 polymorphism of gene was investigated by Polymerase Chain Reaction-Restriction Fragment Length poly morphism technique and the frequency of which was compared between the two groups.

Results: The frequency of TT, TC, and CC genotypes of gene polymorphism in the case group were 9%, 40%, and 51%, respectively, and in the control group were 11%, 38%, and 51%, respectively. There was no significant difference in the genotypes between two groups (p = 0.882).

Conclusion: According to our results, it seems that polymorphism (rs 14035) is not associated with the risk of RPL in this study population.

References

Jung Y, Jeon Y, Rah H, Kim J, Shin J, Choi D . Genetic variants in microRNA machinery genes are associated [corrected] with idiopathic recurrent pregnancy loss risk. PLoS One. 2014; 9(4):e95803. PMC: 4000197. DOI: 10.1371/journal.pone.0095803. View

Arjmand F, Ghasemi N, Mirghanizadeh S, Samadi M . The balance of the immune system between HLA-G and NK cells in unexplained recurrent spontaneous abortion and polymorphisms analysis. Immunol Res. 2016; 64(3):785-90. DOI: 10.1007/s12026-015-8771-9. View

Jeon Y, Choi Y, Rah H, Kim S, Choi D, Cha S . Association study of microRNA polymorphisms with risk of idiopathic recurrent spontaneous abortion in Korean women. Gene. 2012; 494(2):168-73. DOI: 10.1016/j.gene.2011.12.026. View

Sierra S, Stephenson M . Genetics of recurrent pregnancy loss. Semin Reprod Med. 2006; 24(1):17-24. DOI: 10.1055/s-2006-931797. View

Sazer S, Dasso M . The ran decathlon: multiple roles of Ran. J Cell Sci. 2000; 113 ( Pt 7):1111-8. DOI: 10.1242/jcs.113.7.1111. View

Lewis B, Burge C, Bartel D . Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 2005; 120(1):15-20. DOI: 10.1016/j.cell.2004.12.035. View

Choi Y, Kwon H, Kim J, Shin J, Choi Y, Yoon T . Haplotype-based association of ACE I/D, AT1R 1166A>C, and AGT M235T polymorphisms in renin-angiotensin-aldosterone system genes in Korean women with idiopathic recurrent spontaneous abortions. Eur J Obstet Gynecol Reprod Biol. 2011; 158(2):225-8. DOI: 10.1016/j.ejogrb.2011.04.028. View

Moore M, Blobel G . A G protein involved in nucleocytoplasmic transport: the role of Ran. Trends Biochem Sci. 1994; 19(5):211-6. DOI: 10.1016/0968-0004(94)90024-8. View

Ardestani M, Nodushan H, Aflatoonian A, Ghasemi N, Sheikhha M . Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss. Iran J Reprod Med. 2014; 11(1):61-4. PMC: 3941375. View

10.

Zahraei M, Sheikhha M, Kalantar S, Ghasemi N, Jahaninejad T, Rajabi S . The association of arylendosulfatase 1 (SULF1) gene polymorphism with recurrent miscarriage. J Assist Reprod Genet. 2013; 31(2):157-61. PMC: 3933597. DOI: 10.1007/s10815-013-0150-7. View

11.

Rah H, Jeon Y, Lee B, Kim J, Shim S, Lee W . Association of polymorphisms in microRNA machinery genes (DROSHA, DICER1, RAN, and XPO5) with risk of idiopathic primary ovarian insufficiency in Korean women. Menopause. 2013; 20(10):1067-73. DOI: 10.1097/GME.0b013e3182883907. View

12.

Abdollahi E, Tavasolian F, Ghasemi N, Mirghanizadeh S, Azizi M, Ghoryani M . Association between lower frequency of R381Q variant (rs11209026) in IL-23 receptor gene and increased risk of recurrent spontaneous abortion (RSA). J Immunotoxicol. 2015; 12(4):317-21. DOI: 10.3109/1547691x.2014.978056. View

13.

Donker R, Mouillet J, Nelson D, Sadovsky Y . The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Mol Hum Reprod. 2007; 13(4):273-9. DOI: 10.1093/molehr/gam006. View

14.

Chakrabarty A, Tranguch S, Daikoku T, Jensen K, Furneaux H, Dey S . MicroRNA regulation of cyclooxygenase-2 during embryo implantation. Proc Natl Acad Sci U S A. 2007; 104(38):15144-9. PMC: 1986627. DOI: 10.1073/pnas.0705917104. View

15.

OToole A, Miller S, Haines N, Zink M, Serra M . Comprehensive thermodynamic analysis of 3' double-nucleotide overhangs neighboring Watson-Crick terminal base pairs. Nucleic Acids Res. 2006; 34(11):3338-44. PMC: 1500867. DOI: 10.1093/nar/gkl428. View

16.

Fotoohi M, Ghasemi N, Mirghanizadeh S, Vakili M, Samadi M . Association between HLA-E gene polymorphism and unexplained recurrent spontaneous abortion (RSA) in Iranian women. Int J Reprod Biomed. 2016; 14(7):477-82. PMC: 4971558. View

17.

Enquobahrie D, Abetew D, Sorensen T, Willoughby D, Chidambaram K, Williams M . Placental microRNA expression in pregnancies complicated by preeclampsia. Am J Obstet Gynecol. 2010; 204(2):178.e12-21. PMC: 3040986. DOI: 10.1016/j.ajog.2010.09.004. View

18.

Tabibnejad N, Sheikhha M, Ghasemi N, Fesahat F, Soleimani M, Aflatoonian A . Association between early embryo morphokinetics plus cumulus cell gene expression and assisted reproduction outcomes in polycystic ovary syndrome women. Reprod Biomed Online. 2018; 38(2):139-151. DOI: 10.1016/j.rbmo.2018.10.010. View

19.

Fu M, Xu W, Qin T, Xu K . [The Association Between the Polymorphisms of miRNA Biogenesis Related Genes( and )and Unexplained Recurrent Spontaneous Abortion in Chinese Women]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2017; 48(6):880-885. View

20.

Kotlabova K, Doucha J, Hromadnikova I . Placental-specific microRNA in maternal circulation--identification of appropriate pregnancy-associated microRNAs with diagnostic potential. J Reprod Immunol. 2011; 89(2):185-91. DOI: 10.1016/j.jri.2011.02.006. View