Placental MicroRNA Expression in Pregnancies Complicated by Preeclampsia

Overview

Journal Am J Obstet Gynecol

Publisher Elsevier

Specialty Gynecology & Obstetrics

Date 2010 Nov 25

PMID 21093846

Citations 109

Authors

Daniel A Enquobahrie

Dejene F Abetew

Tanya K Sorensen

David Willoughby

Kumaravel Chidambaram

Michelle A Williams

Affiliations

Soon will be listed here.

Abstract

Objective: The role of posttranscription regulation in preeclampsia is largely unknown. We investigated preeclampsia-related placental microRNA (miRNA) expression using microarray and confirmatory quantitative real-time polymerase chain reaction experiments.

Study Design: Placental expressions of characterized and novel miRNAs (1295 probes) were measured in samples collected from 20 preeclampsia cases and 20 controls. Differential expression was evaluated using Student t test and fold change analyses. In pathway analysis, we examined functions/functional relationships of targets of differentially expressed miRNAs.

Results: Eight miRNAs were differentially expressed (1 up-regulated and 7 down-regulated) among preeclampsia cases compared with controls. These included previously identified candidates (miR-210, miR-1, and a miRNA in the 14q32.31 cluster region) and others that are novel (miR-584 and miR-34c-5p). These miRNAs target genes that participate in organ/system development (cardiovascular and reproductive system), immunologic dysfunction, cell adhesion, cell cycle, and signaling.

Conclusion: Expression of miRNAs that target genes in diverse pathophysiological processes is altered in the setting of preeclampsia.

Citing Articles

Lateral Flow Assay for Preeclampsia Screening Using DNA Hairpins and Surface-Enhanced Raman-Active Nanoprobes Targeting hsa-miR-17-5p.

Ng K, Jaitpal S, Vu N, San Juan A, Tripathy S, Kodam R Biosensors (Basel). 2024; 14(11).

PMID: 39589994 PMC: 11592307. DOI: 10.3390/bios14110535.

Integrated analysis of microRNA and mRNA expression profiles in Preeclampsia.

Ping Z, Feng Y, Lu Y, Ai L, Jiang H BMC Med Genomics. 2023; 16(1):309.

PMID: 38041082 PMC: 10691005. DOI: 10.1186/s12920-023-01740-3.

MicroRNA-206 Contributes to the Progression of Preeclampsia by Suppressing the Viability and Mobility of Trophocytes via the Inhibition of AGTR1.

Mo W, Jin J, Wang X, Luan W, Yan J, Long X Physiol Res. 2023; 72(5):597-606.

PMID: 38015759 PMC: 10751052. DOI: 10.33549/physiolres.935131.

Trophoblast Cell Function in the Antiphospholipid Syndrome.

Vrzic Petronijevic S, Vilotic A, Bojic-Trbojevic Z, Kostic S, Petronijevic M, Vicovac L Biomedicines. 2023; 11(10).

PMID: 37893055 PMC: 10604227. DOI: 10.3390/biomedicines11102681.

Seminar: Extracellular Vesicles as Mediators of Environmental Stress in Human Disease.

Kalia V, Baccarelli A, Happel C, Hollander J, Jukic A, McAllister K Environ Health Perspect. 2023; 131(10):104201.

PMID: 37861803 PMC: 10588739. DOI: 10.1289/EHP12980.

References

Grill S, Rusterholz C, Zanetti-Dallenbach R, Tercanli S, Holzgreve W, Hahn S . Potential markers of preeclampsia--a review. Reprod Biol Endocrinol. 2009; 7:70. PMC: 2717076. DOI: 10.1186/1477-7827-7-70. View

Corney D, Flesken-Nikitin A, Godwin A, Wang W, Nikitin A . MicroRNA-34b and MicroRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth. Cancer Res. 2007; 67(18):8433-8. DOI: 10.1158/0008-5472.CAN-07-1585. View

Saito S, Shiozaki A, Nakashima A, Sakai M, Sasaki Y . The role of the immune system in preeclampsia. Mol Aspects Med. 2007; 28(2):192-209. DOI: 10.1016/j.mam.2007.02.006. View

Eickhoff B, Korn B, Schick M, Poustka A, van der Bosch J . Normalization of array hybridization experiments in differential gene expression analysis. Nucleic Acids Res. 1999; 27(22):e33. PMC: 148709. DOI: 10.1093/nar/27.22.e33. View

Redman C, Sargent I . Latest advances in understanding preeclampsia. Science. 2005; 308(5728):1592-4. DOI: 10.1126/science.1111726. View

Eisen M, Spellman P, Brown P, Botstein D . Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A. 1998; 95(25):14863-8. PMC: 24541. DOI: 10.1073/pnas.95.25.14863. View

Huppertz B . Placental origins of preeclampsia: challenging the current hypothesis. Hypertension. 2008; 51(4):970-5. DOI: 10.1161/HYPERTENSIONAHA.107.107607. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Mattick J, Makunin I . Non-coding RNA. Hum Mol Genet. 2006; 15 Spec No 1:R17-29. DOI: 10.1093/hmg/ddl046. View

10.

Bartel D . MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116(2):281-97. DOI: 10.1016/s0092-8674(04)00045-5. View

11.

Vaiman D, Mondon F, Garces-Duran A, Mignot T, Robert B, Rebourcet R . Hypoxia-activated genes from early placenta are elevated in preeclampsia, but not in Intra-Uterine Growth Retardation. BMC Genomics. 2005; 6:111. PMC: 1236921. DOI: 10.1186/1471-2164-6-111. View

12.

Liao Y, Lonnerdal B . miR-584 mediates post-transcriptional expression of lactoferrin receptor in Caco-2 cells and in mouse small intestine during the perinatal period. Int J Biochem Cell Biol. 2009; 42(8):1363-9. DOI: 10.1016/j.biocel.2009.07.019. View

13.

Zhang C . MicroRNomics: a newly emerging approach for disease biology. Physiol Genomics. 2008; 33(2):139-47. DOI: 10.1152/physiolgenomics.00034.2008. View

14.

Zhu X, Han T, Sargent I, Yin G, Yao Y . Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies. Am J Obstet Gynecol. 2009; 200(6):661.e1-7. DOI: 10.1016/j.ajog.2008.12.045. View

15.

Urbich C, Kuehbacher A, Dimmeler S . Role of microRNAs in vascular diseases, inflammation, and angiogenesis. Cardiovasc Res. 2008; 79(4):581-8. DOI: 10.1093/cvr/cvn156. View

16.

. Report of the National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy. Am J Obstet Gynecol. 2000; 183(1):S1-S22. View

17.

Suzuki Y, Lopez V, Lonnerdal B . Mammalian lactoferrin receptors: structure and function. Cell Mol Life Sci. 2005; 62(22):2560-75. PMC: 11139119. DOI: 10.1007/s00018-005-5371-1. View

18.

Ikeda S, He A, Kong S, Lu J, Bejar R, Bodyak N . MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes. Mol Cell Biol. 2009; 29(8):2193-204. PMC: 2663304. DOI: 10.1128/MCB.01222-08. View

19.

Enquobahrie D, Meller M, Rice K, Psaty B, Siscovick D, Williams M . Differential placental gene expression in preeclampsia. Am J Obstet Gynecol. 2008; 199(5):566.e1-11. PMC: 2702488. DOI: 10.1016/j.ajog.2008.04.020. View

20.

Fasanaro P, DAlessandra Y, Stefano V, Melchionna R, Romani S, Pompilio G . MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand Ephrin-A3. J Biol Chem. 2008; 283(23):15878-83. PMC: 3259646. DOI: 10.1074/jbc.M800731200. View