Ultrastructural Localization of Osteocalcin in Rat Tooth Germs by Immunogold Staining

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1988 Jan 1

PMID 3262606

Citations 2

Authors

I Gorter de Vries

D Coomans

E Wisse

Affiliations

Soon will be listed here.

Abstract

Osteocalcin was localized by indirect immunogold staining of thin frozen sections of rat tooth germs which had been fixed by different methods. Acrolein fixation proved to be satisfactory considering the preservation of fine structure and antigenicity. In odontoblasts, osteocalcin was found to be localized in the cisternae of the rough endoplasmic reticulum and Golgi apparatus. Few positive transport vesicles were found. Staining for osteocalcin in odontoblastic processes was only observed after strong fixation and was intense in odontoblasts engaged in early dentine formation. Predentine was slightly positive in the neighbourhood of positive processes. Matrix vesicles were negative and strong osteocalcin labeling of dentine seemed to appear after the onset of mineralization.

Citing Articles

Ultrastructural localization of dentine phosphoprotein in rat tooth germs by immunogold staining.

Gorter de Vries I, Wisse E Histochemistry. 1989; 91(1):69-75.

PMID: 2925450 DOI: 10.1007/BF00501914.

Effect of warfarin on early rat tooth development.

Gorter de Vries I, Wisse E, Williamson M, Price P Calcif Tissue Int. 1991; 49(5):355-8.

PMID: 1782575 DOI: 10.1007/BF02556259.

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