Ultrastructural Localization of Dentine Phosphoprotein in Rat Tooth Germs by Immunogold Staining

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1989 Jan 1

PMID 2925450

Citations 1

Authors

I Gorter de Vries

E Wisse

Affiliations

Soon will be listed here.

Abstract

Dentine phosphoprotein (DPP) was localized on thin frozen sections of fixed rat tooth germs by indirect immunogold staining. Antisera were directed against DPP and against glutaraldehyde-treated DPP and were characterized by immuno-electroblotting. In odontoblasts, DPP was found to be localized in the cisternae of the rough endoplasmic reticulum (RER) and the Golgi apparatus and in Golgi-associated vesicles. Odontoblastic processes were moderately positive for DPP and dentine was intensely labeled on frozen sections of unfixed tissue. Predentine showed a slight immunoreactivity. These results indicate the synthesis of DPP in the RER, its accumulation in the Golgi apparatus and its vesicular transport and secretion via the odontoblastic processes into dentine. The close association of the gold particles with the dentinal collagen fibres makes a role of DPP in linking mineral to collagen conceivable. Matrix vesicles were negative for DPP, suggesting that the protein is not present at the sites of matrix vesicle-associated nucleation.

Citing Articles

Sialylation of terminal saccharides of glycoconjugates expressed by murine molar tooth germs developing in vitro and in vivo.

Jowett A, Kimber S, Ferguson M J Anat. 1994; 185 ( Pt 1):85-94.

PMID: 7559118 PMC: 1166817.

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