PCDH7 Interacts with GluN1 and Regulates Dendritic Spine Morphology and Synaptic Function

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jul 4

PMID 32616769

Citations 12

Authors

Yuanyuan Wang

Meghan Kerrisk Campbell

Irene Tom

Oded Foreman

Jesse E Hanson

Morgan Sheng

Affiliations

Soon will be listed here.

Abstract

The N-terminal domain (NTD) of the GluN1 subunit (GluN1-NTD) is important for NMDA receptor structure and function, but the interacting proteins of the GluN1-NTD are not well understood. Starting with an unbiased screen of ~ 1,500 transmembrane proteins using the purified GluN1-NTD protein as a bait, we identify Protocadherin 7 (PCDH7) as a potential interacting protein. PCDH7 is highly expressed in the brain and has been linked to CNS disorders, including epilepsy. Using primary neurons and brain slice cultures, we find that overexpression and knockdown of PCDH7 induce opposing morphological changes of dendritic structures. We also find that PCDH7 overexpression reduces synaptic NMDA receptor currents. These data show that PCDH7 can regulate dendritic spine morphology and synaptic function, possibly via interaction with the GluN1 subunit.

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