LRIK Interacts with the Ku70-Ku80 Heterodimer Enhancing the Efficiency of NHEJ Repair

Overview

Journal Cell Death Differ

Specialty Cell Biology

Date 2020 Jun 27

PMID 32587379

Citations 14

Authors

Dan Wang

Zheng Zhou

Erzhong Wu

Can Ouyang

Guifeng Wei

Yunfei Wang

Dandan He

Ya Cui

Dongdong Zhang

Xiaomin Chen

Simon H Reed

Jianjun Luo

Runsheng Chen

Affiliations

Soon will be listed here.

Abstract

Despite recent advances in our understanding of the function of long noncoding RNAs (lncRNAs), their roles and functions in DNA repair pathways remain poorly understood. By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), we identified a novel lncRNA referred to as LRIK that interacts with Ku, which enhances the ability of the Ku heterodimer to detect the presence of DSBs. Here, we show that depletion of LRIK generates significantly enhanced sensitivity to DSB-inducing agents and reduced DSB repair efficiency. In response to DSBs, LRIK enhances the recruitment of repair factors at DSB sites and facilitates γH2AX signaling. Our results demonstrate that LRIK is necessary for efficient repairing DSBs via nonhomologous end-joining pathway.

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