SCYL1 Arginine Methylation by PRMT1 is Essential for Neurite Outgrowth Via Golgi Morphogenesis

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2020 Jun 26

PMID 32583741

Citations 9

Authors

Genki Amano

Shinsuke Matsuzaki

Yasutake Mori

Ko Miyoshi

Sarina Han

Sho Shikada

Hironori Takamura

Takeshi Yoshimura

Taiichi Katayama

Affiliations

Soon will be listed here.

Abstract

Arginine methylation is a common posttranslational modification that modulates protein function. SCY1-like pseudokinase 1 (SCYL1) is crucial for neuronal functions and interacts with γ-COP to form coat protein complex I (COPI) vesicles that regulate Golgi morphology. However, the molecular mechanism by which SCYL1 is regulated remains unclear. Here, we report that the γ-COP-binding site of SCYL1 is arginine-methylated by protein arginine methyltransferase 1 (PRMT1) and that SCYL1 arginine methylation is important for the interaction of SCYL1 with γ-COP. PRMT1 was colocalized with SCYL1 in the Golgi fraction. Inhibition of PRMT1 suppressed axon outgrowth and dendrite complexity via abnormal Golgi morphology. Knockdown of SCYL1 by small interfering RNA (siRNA) inhibited axon outgrowth, and the inhibitory effect was rescued by siRNA-resistant SCYL1, but not SCYL1 mutant, in which the arginine methylation site was replaced. Thus, PRMT1 regulates Golgi morphogenesis via SCYL1 arginine methylation. We propose that SCYL1 arginine methylation by PRMT1 contributes to axon and dendrite morphogenesis in neurons.

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