Long Non-coding RNA and Its Molecular Mechanisms in Polycystic Ovary Syndrome

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2020 Jun 20

PMID 32559120

Citations 7

Authors

Ying Li

Jun Zhang

Yu-Dong Liu

Xing-Yu Zhou

Xin Chen

Jing Zhe

Qing-Yan Zhang

Xiao-Fei Zhang

Ying-Xue Chen

Zhe Wang

Shi-Ling Chen

Affiliations

Soon will be listed here.

Abstract

Polycystic ovary syndrome (PCOS) causes anovulatory infertility in women of reproductive age, but etiopathogenesis of PCOS remains undetermined. Taurine up-regulated 1 (), an evolutionarily conserved long non-coding RNA, performs various biological functions; however, the role of in PCOS remains unclear. Herein, expression was assayed in granulosa cells (GCs) of 100 patients with PCOS and 100 control participants. Receiver operating characteristic (ROC) curve analysis was conducted to determine the diagnostic value of in PCOS. expression was also silenced in KGN cells to explore the role of in cellular proliferation, apoptosis, cell-cycle progression, autophagy, and steroidogenesis. We found that levels were dramatically increased in the PCOS group compared with those of the control group; this increased expression was related to a rising antral follicle count (R = 0.209, P < 0.001 versus control). The ROC curve indicated a significant separation between PCOS group and the control group (AUC: 0.702; 95% CI: 0.630-0.773; P < 0.001). showed a predominantly nuclear localization in human GCs. knockdown reduced cellular proliferation, and promoted MAPKs pathway-dependent apoptosis and P21-dependent autophagy, but may not affect cell-cycle progression. knockdown increased aromatase expression and oestradiol biosynthesis. Our results indicate that increased expression in PCOS GCs may contribute to excessive follicular activation and growth, and may disrupt the selection of dominant follicle. Our study shows that can be used as a diagnostic biomarker for PCOS.

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