Serial Scanning Electron Microscopy of Anti-PKHD1L1 Immuno-gold Labeled Mouse Hair Cell Stereocilia Bundles

Overview

Journal Sci Data

Specialty Science

Date 2020 Jun 20

PMID 32555200

Citations 4

Authors

Maryna V Ivanchenko

Marcelo Cicconet

Hoor Al Jandal

Xudong Wu

David P Corey

Artur A Indzhykulian

Affiliations

Soon will be listed here.

Abstract

Serial electron microscopy techniques have proven to be a powerful tool in biology. Unfortunately, the data sets they generate lack robust and accurate automated segmentation algorithms. In this data descriptor publication, we introduce a serial focused ion beam scanning electron microscopy (FIB-SEM) dataset consisting of six outer hair cell (OHC) stereocilia bundles, and the supranuclear part of the hair cell bodies. Also presented are the manual segmentations of stereocilia bundles and the gold bead labeling of PKHD1L1, a coat protein of hair cell stereocilia important for hearing in mice. This depository includes all original data and several intermediate steps of the manual analysis, as well as the MATLAB algorithm used to generate a three-dimensional distribution map of gold labels. They serve as a reference dataset, and they enable reproduction of our analysis, evaluation and improvement of current methods of protein localization, and training of algorithms for accurate automated segmentation.

Citing Articles

VASCilia (Vision Analysis StereoCilia): A Napari Plugin for Deep Learning-Based 3D Analysis of Cochlear Hair Cell Stereocilia Bundles.

Kassim Y, Rosenberg D, Das S, Huang Z, Rahman S, Shammaa I bioRxiv. 2024; .

PMID: 38948743 PMC: 11212889. DOI: 10.1101/2024.06.17.599381.

Mini-PCDH15 gene therapy rescues hearing in a mouse model of Usher syndrome type 1F.

Ivanchenko M, Hathaway D, Klein A, Pan B, Strelkova O, De-la-Torre P Nat Commun. 2023; 14(1):2400.

PMID: 37100771 PMC: 10133396. DOI: 10.1038/s41467-023-38038-y.

Electron Microscopy Techniques for Investigating Structure and Composition of Hair-Cell Stereociliary Bundles.

Ivanchenko M, Indzhykulian A, Corey D Front Cell Dev Biol. 2021; 9:744248.

PMID: 34746139 PMC: 8569945. DOI: 10.3389/fcell.2021.744248.

Serial scanning electron microscopy of anti-PKHD1L1 immuno-gold labeled mouse hair cell stereocilia bundles.

Ivanchenko M, Cicconet M, Al Jandal H, Wu X, Corey D, Indzhykulian A Sci Data. 2020; 7(1):182.

PMID: 32555200 PMC: 7299942. DOI: 10.1038/s41597-020-0509-4.

References

Indzhykulian A, Stepanyan R, Nelina A, Spinelli K, Ahmed Z, Belyantseva I . Molecular remodeling of tip links underlies mechanosensory regeneration in auditory hair cells. PLoS Biol. 2013; 11(6):e1001583. PMC: 3679001. DOI: 10.1371/journal.pbio.1001583. View

Kazmierczak P, Sakaguchi H, Tokita J, Wilson-Kubalek E, Milligan R, Muller U . Cadherin 23 and protocadherin 15 interact to form tip-link filaments in sensory hair cells. Nature. 2007; 449(7158):87-91. DOI: 10.1038/nature06091. View

Hasson T, Gillespie P, Garcia J, Macdonald R, Zhao Y, Yee A . Unconventional myosins in inner-ear sensory epithelia. J Cell Biol. 1997; 137(6):1287-307. PMC: 2132524. DOI: 10.1083/jcb.137.6.1287. View

Michel V, Goodyear R, Weil D, Marcotti W, Perfettini I, Wolfrum U . Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells. Dev Biol. 2005; 280(2):281-94. DOI: 10.1016/j.ydbio.2005.01.014. View

Andrade L . Evidence for changes in beta- and gamma-actin proportions during inner ear hair cell life. Cytoskeleton (Hoboken). 2015; 72(6):282-91. PMC: 4529820. DOI: 10.1002/cm.21227. View

Katsuno T, Belyantseva I, Cartagena-Rivera A, Ohta K, Crump S, Petralia R . TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing. JCI Insight. 2019; 4(12). PMC: 6629139. DOI: 10.1172/jci.insight.128561. View

Chen Q, Mahendrasingam S, Tickle J, Hackney C, Furness D, Fettiplace R . The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells. Eur J Neurosci. 2012; 36(3):2302-10. PMC: 3412916. DOI: 10.1111/j.1460-9568.2012.08159.x. View

Siemens J, Lillo C, Dumont R, Reynolds A, Williams D, Gillespie P . Cadherin 23 is a component of the tip link in hair-cell stereocilia. Nature. 2004; 428(6986):950-5. DOI: 10.1038/nature02483. View

Garcia J, Yee A, Gillespie P, Corey D . Localization of myosin-Ibeta near both ends of tip links in frog saccular hair cells. J Neurosci. 1998; 18(21):8637-47. PMC: 6793541. View

10.

Hackney C, Mahendrasingam S, Jones E, Fettiplace R . The distribution of calcium buffering proteins in the turtle cochlea. J Neurosci. 2003; 23(11):4577-89. PMC: 6740801. View

11.

Frolenkov G, Belyantseva I, Friedman T, Griffith A . Genetic insights into the morphogenesis of inner ear hair cells. Nat Rev Genet. 2004; 5(7):489-98. DOI: 10.1038/nrg1377. View

12.

Goodyear R, Marcotti W, Kros C, Richardson G . Development and properties of stereociliary link types in hair cells of the mouse cochlea. J Comp Neurol. 2005; 485(1):75-85. DOI: 10.1002/cne.20513. View

13.

Ivanchenko M, Cicconet M, Al Jandal H, Wu X, Corey D, Indzhykulian A . Serial scanning electron microscopy of anti-PKHD1L1 immuno-gold labeled mouse hair cell stereocilia bundles. Sci Data. 2020; 7(1):182. PMC: 7299942. DOI: 10.1038/s41597-020-0509-4. View

14.

Grillet N, Xiong W, Reynolds A, Kazmierczak P, Sato T, Lillo C . Harmonin mutations cause mechanotransduction defects in cochlear hair cells. Neuron. 2009; 62(3):375-87. PMC: 2691393. DOI: 10.1016/j.neuron.2009.04.006. View

15.

Beurg M, Fettiplace R, Nam J, Ricci A . Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging. Nat Neurosci. 2009; 12(5):553-8. PMC: 2712647. DOI: 10.1038/nn.2295. View

16.

Bullen A, West T, Moores C, Ashmore J, Fleck R, MacLellan-Gibson K . Association of intracellular and synaptic organization in cochlear inner hair cells revealed by 3D electron microscopy. J Cell Sci. 2015; 128(14):2529-40. PMC: 4510854. DOI: 10.1242/jcs.170761. View

17.

Avan P, Le Gal S, Michel V, Dupont T, Hardelin J, Petit C . Otogelin, otogelin-like, and stereocilin form links connecting outer hair cell stereocilia to each other and the tectorial membrane. Proc Natl Acad Sci U S A. 2019; 116(51):25948-25957. PMC: 6926040. DOI: 10.1073/pnas.1902781116. View

18.

Delling M, Indzhykulian A, Liu X, Li Y, Xie T, Corey D . Primary cilia are not calcium-responsive mechanosensors. Nature. 2016; 531(7596):656-60. PMC: 4851444. DOI: 10.1038/nature17426. View

19.

Zeng W, Grillet N, Dewey J, Trouillet A, Krey J, Barr-Gillespie P . Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function. J Neurosci. 2016; 36(35):9201-16. PMC: 5005726. DOI: 10.1523/JNEUROSCI.0093-16.2016. View

20.

Andrade L, Salles F, Grati M, Manor U, Kachar B . Tectorins crosslink type II collagen fibrils and connect the tectorial membrane to the spiral limbus. J Struct Biol. 2016; 194(2):139-46. PMC: 4805521. DOI: 10.1016/j.jsb.2016.01.006. View