Combined Analysis of DNA Methylome and Transcriptome Reveal Novel Candidate Genes Related to Porcine F4ab/ac-Induced Diarrhea

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2020 Jun 18

PMID 32547963

Citations 4

Authors

Wenwen Wang

Chuanli Zhou

Hui Tang

Ying Yu

Qin Zhang

Affiliations

Soon will be listed here.

Abstract

Enterotoxigenic (ETEC) that express F4 (K88) fimbriae are the principal microorganisms responsible for bacterial diarrhea in neonatal and pre-weaning piglets. To better understand the molecular effects of ETEC F4ab/ac infection, we performed a genome-wide comparison of the changes in DNA methylation and gene expression in ETEC F4ab/ac infected porcine intestinal epithelial cells. We characterized the pattern of changes in methylation and found 3297 and 1593 differentially methylated regions in cells infected with F4ab and F4ac, respectively. Moreover, 606 and 780 differentially expressed genes (DEGs) in ETEC F4ab and F4ac infected cells were detected and these genes were highly enriched in immune/defense response related pathways. Integrative analysis identified 27 and 10 genes showing inverse correlations between promoter methylation and expression with ETEC F4ab/ac infection. Altered DNA methylation and expression of various genes suggested their roles and potential functional interactions upon ETEC F4ab/ac infection. Further functional analyses revealed that three DEGs (, and ) in F4ab infected cells and three DEGs (, and ) in F4ac infected cells are likely involved in the host cells response to ETEC infection. Our data provides further insight into the epigenetic and transcriptomic alterations of ETEC F4ab/ac infected porcine intestinal epithelial cells, and may advance the identification of biomarkers and drug targets for predicting susceptibility to and controlling ETEC F4ab/ac induced diarrhea.

Citing Articles

Identification of a 5-Methylcytosine Site (mC-7) That May Inhibit Expression and Regulate F18 Susceptibility in IPEC-J2 Cells.

Shi X, Yu L, Huang R, Bao W, Wu S, Wu Z Vet Sci. 2022; 9(11).

PMID: 36356076 PMC: 9698616. DOI: 10.3390/vetsci9110600.

Combined Analysis of RRBS DNA Methylome and Transcriptome Reveal Novel Candidate Genes Related to Porcine Type C-Induced Diarrhea.

Huang X, Yang Q, Yan Z, Wang P, Shi H, Li J Front Genet. 2022; 13:803477.

PMID: 35401691 PMC: 8990837. DOI: 10.3389/fgene.2022.803477.

Sodium Humate Alleviates Enterotoxigenic -Induced Intestinal Dysfunction via Alteration of Intestinal Microbiota and Metabolites in Mice.

Wang D, He Y, Liu K, Deng S, Fan Y, Liu Y Front Microbiol. 2022; 13:809086.

PMID: 35401451 PMC: 8992542. DOI: 10.3389/fmicb.2022.809086.

Transcriptome Analysis Identifies Strategies Targeting Immune Response-Related Pathways to Control Enterotoxigenic Infection in Porcine Intestinal Epithelial Cells.

Wu Q, Cui D, Chao X, Chen P, Liu J, Wang Y Front Vet Sci. 2021; 8:677897.

PMID: 34447800 PMC: 8383179. DOI: 10.3389/fvets.2021.677897.

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