Emulsified Silicone Oil is Taken Up by and Induces Pro-inflammatory Response in Primary Retinal Microglia

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 2020 Jun 6

PMID 32500303

Citations 7

Authors

Alexa Klettner

Antonia Harms

Vicki Waetzig

Jan Tode

Konstantine Purtskhvanidze

Johann Roider

Affiliations

Soon will be listed here.

Abstract

Purpose: Silicone oil is used as endotamponade in combination with vitrectomy. Thinning of retinal layers and loss of retinal cells under silicone oil use have been found. Here, we investigate the influence of silicone oil on primary microglia cells.

Methods: Primary microglia cells were prepared from the porcine retina. Microglia identity was assessed with Iba1 staining. Silicone oil was emulsified by sonification. Cell morphology and silicone oil uptake were evaluated by light microscopy after Coomassie blue staining. Cytokine secretion was evaluated with ELISA. Toxicity of silicone oil on microglia and toxic effect of silicone oil-treated microglia on neuronal cell line PC12 were evaluated by MTT or WST assay, respectively.

Results: Microglia took up silicone oil droplets after 72 h of incubation. Silicone oil induced no toxicity but increased the metabolism in microglial cells. In addition, the secretion of IL-6 and IL-8, but not of IL-1ß or TNF-α, was induced. Silicone oil-treated microglia did not exert any neurotoxic effect on differentiated PC12 cells but induced an increase in metabolism.

Conclusion: Emulsified silicone oil changes the activity level of microglia and induces the secretion of IL-6 and IL-8. Neurotoxicity is not induced. Further experiments are required to investigate the long-term effect of silicone oil on microglia and their consequent effect on neuronal cells.

Citing Articles

Prognostic Factors for Recurrent Rhegmatogenous Retinal Detachment after Silicone Oil Removal.

Park H, Kim M, Kim S, Kang H Korean J Ophthalmol. 2024; 38(4):284-295.

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Effects of Fucoidans on Activated Retinal Microglia.

Dorschmann P, Hunger F, Schroth H, Chen S, Kopplin G, Roider J Int J Mol Sci. 2024; 25(11).

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Silicone Oil Tamponade Removal: Which Technique Is More Effective? An X-Ray Photoemission Spectroscopy Study.

Rossi T, Canepa P, Cavalleri O, Rolandi R, Querzoli G, DAgostino I Transl Vis Sci Technol. 2023; 12(4):21.

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Retinal Nerve Fiber Layer Changes after Intraocular Silicone Oil Tamponade in Rhegmatogenous Retinal Detachment.

Chikmah F, Ichsan A, Islam I, Hendarto J, Muhiddin H, Budu Vision (Basel). 2023; 7(1).

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[Visual acuity reduction and silicone oil tamponade].

Wolf A, Romano M, Haritoglou C Ophthalmologie. 2022; 119(8):781-788.

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