Continuous Cell Lines from the European Biting Midge (Meigen, 1830)

Overview

Journal Microorganisms

Specialty Microbiology

Date 2020 Jun 4

PMID 32486323

Citations 6

Authors

Lesley Bell-Sakyi

Fauziah Mohd Jaafar

Baptiste Monsion

Lisa Luu

Eric Denison

Simon Carpenter

Houssam Attoui

Peter P C Mertens

Affiliations

Soon will be listed here.

Abstract

biting midges (Diptera: Ceratopogonidae) transmit arboviruses of veterinary or medical importance, including bluetongue virus (BTV) and Schmallenberg virus, as well as causing severe irritation to livestock and humans. Arthropod cell lines are essential laboratory research tools for the isolation and propagation of vector-borne pathogens and the investigation of host-vector-pathogen interactions. Here we report the establishment of two continuous cell lines, CNE/LULS44 and CNE/LULS47, from embryos of , a midge distributed throughout the Western Palearctic region. Species origin of the cultured cells was confirmed by polymerase chain reaction (PCR) amplification and sequencing of a fragment of the gene, and the absence of bacterial contamination was confirmed by bacterial 16S rRNA PCR. Both lines have been successfully cryopreserved and resuscitated. The majority of cells examined in both lines had the expected diploid chromosome number of 2 = 6. Transmission electron microscopy of CNE/LULS44 cells revealed the presence of large mitochondria within cells of a diverse population, while arrays of virus-like particles were not seen. CNE/LULS44 cells supported replication of a strain of BTV serotype 1, but not of a strain of serotype 26 which is not known to be insect-transmitted. These new cell lines will expand the scope of research on -borne pathogens.

Citing Articles

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Identification of the Genome Segments of Bluetongue Virus Type 26/Type 1 Reassortants Influencing Horizontal Transmission in a Mouse Model.

Attoui H, Monsion B, Klonjkowski B, Zientara S, Mertens P, Mohd Jaafar F Viruses. 2021; 13(11).

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Isolation in Natural Host Cell Lines of Strains Pip from the Mosquito and Pap from the Sand Fly .

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