» Articles » PMID: 2022869

A RNA Virus in Cells from Culicoides Variipennis

Overview
Specialty Biology
Date 1991 Mar 1
PMID 2022869
Citations 17
Authors
Affiliations
Soon will be listed here.
Abstract

A virus was detected in cells (designated CuVa) cultured from one laboratory colony of the biting midge, Culicoides variipennis. By electron microscopy (30 nm), nonenveloped, icosahedral virions arranged separately and in crystalline matrix arrays were seen in the cytoplasm but not in the nucleus of CuVa cells. Separation by 10% polyacrylamide gel electrophoresis revealed multiple bands of viral-induced double-stranded RNA. Inoculation of this virus onto different cell lines and intracranially into suckling mice revealed no detectable pathology. Immunoperoxidase staining using polyclonal antibody determined that the virus is infectious to toad cells, bovine endothelial cells, bovine kidney cells, mosquito cells, and cells (designated KC) initiated from another laboratory colony of C. variipennis. KC cells infected with this virus were coinfected with bluetongue virus with no decrease in bluetongue virus titer.

Citing Articles

Emergence of Bluetongue Virus Serotype 3, the Netherlands, September 2023.

Holwerda M, Santman-Berends I, Harders F, Engelsma M, Vloet R, Dijkstra E Emerg Infect Dis. 2024; 30(8):1552-1561.

PMID: 38941965 PMC: 11286052. DOI: 10.3201/eid3008.231331.


Orbivirus NS4 Proteins Play Multiple Roles to Dampen Cellular Responses.

Mohd Jaafar F, Belhouchet M, Monsion B, Bell-Sakyi L, Mertens P, Attoui H Viruses. 2023; 15(9).

PMID: 37766314 PMC: 10535134. DOI: 10.3390/v15091908.


Characterization of Epizootic Hemorrhagic Disease Virus Serotype 8 in Naturally Infected Barbary Deer () and (Diptera: Ceratopogonidae) in Tunisia.

Thabet S, Sghaier S, Ben Hassine T, Slama D, Ben Osmane R, Ben Omrane R Viruses. 2023; 15(7).

PMID: 37515253 PMC: 10383031. DOI: 10.3390/v15071567.


Uncovering the Underlying Mechanisms Blocking Replication of Bluetongue Virus Serotype 26 (BTV-26) in Cells.

Monsion B, Mohd Jaafar F, Mertens P, Attoui H Biomolecules. 2023; 13(6).

PMID: 37371457 PMC: 10296729. DOI: 10.3390/biom13060878.


Identification of the Genome Segments of Bluetongue Virus Type 26/Type 1 Reassortants Influencing Horizontal Transmission in a Mouse Model.

Attoui H, Monsion B, Klonjkowski B, Zientara S, Mertens P, Mohd Jaafar F Viruses. 2021; 13(11).

PMID: 34835014 PMC: 8620829. DOI: 10.3390/v13112208.