MEIOK21: a New Component of Meiotic Recombination Bridges Required for Spermatogenesis

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2020 May 29

PMID 32463460

Citations 16

Authors

Yongliang Shang

Tao Huang

Hongbin Liu

Yanlei Liu

Heng Liang

Xiaoxia Yu

Mengjing Li

Binyuan Zhai

Xiao Yang

Yudong Wei

Guoqiang Wang

Zijiang Chen

Shunxin Wang

Liangran Zhang

Affiliations

Soon will be listed here.

Abstract

Repair of DNA double-strand breaks (DSBs) with homologous chromosomes is a hallmark of meiosis that is mediated by recombination 'bridges' between homolog axes. This process requires cooperation of DMC1 and RAD51 to promote homology search and strand exchange. The mechanism(s) regulating DMC1/RAD51-ssDNA nucleoprotein filament and the components of 'bridges' remain to be investigated. Here we show that MEIOK21 is a newly identified component of meiotic recombination bridges and is required for efficient formation of DMC1/RAD51 foci. MEIOK21 dynamically localizes on chromosomes from on-axis foci to 'hanging foci', then to 'bridges', and finally to 'fused foci' between homolog axes. Its chromosome localization depends on DSBs. Knockout of Meiok21 decreases the numbers of HSF2BP and DMC1/RAD51 foci, disrupting DSB repair, synapsis and crossover recombination and finally causing male infertility. Therefore, MEIOK21 is a novel recombination factor and probably mediates DMC1/RAD51 recruitment to ssDNA or their stability on chromosomes through physical interaction with HSF2BP.

Citing Articles

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