Induction of Extrinsic and Intrinsic Apoptosis in Cervical Cancer Cells by Mediated Gold Nanoparticles

Overview

Journal IET Nanobiotechnol

Publisher Wiley

Specialty Biotechnology

Date 2020 May 21

PMID 32433036

Citations 3

Authors

Lavudya Srinivas Naik

Chinnapaka Venkata Ramana Devi

Affiliations

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Abstract

Bio-fabrication of gold nanoparticles (AuNPs) has several advantages like biocompatibility, less toxicity, and eco-friendly in nature over their chemical and physical methods. Currently, the authors fabricated AuNPs using aqueous root extract of () and explored their anticancer application with mechanistic approaches. Different biophysical techniques such as UV-visible spectroscopy, Fourier transform infrared, X-ray diffraction, transmission electron microscopy, selected area electron diffraction, and dynamic light scattering were employed for AuNPs characterisation. The synthesised AuNPs were mono-dispersed, crystalline in nature, anionic surface (-23.9 mV), and spherical particle of an average diameter of 9.4 nm. In addition, the AuNPs were stable in buffers solutions and also biocompatible towards normal human cells (human vascular endothelial cells and human lung cells). The AuNPs were exhibited anticancer activity against different cancer cell lines such as human breast cancer cells, human cervical cancer cells (HeLa) and human lung cancer cells. Further, the pro-apoptotic genes such as Bcl were down-regulated and BAX, Caspase-3, -8, and -9 were up-regulated in HeLa cells as compared to untreated cells. Annexin-V-FITC assay results showed that the AuNPs were induced apoptosis by accumulation of intracellular reactive oxygen species. To their knowledge, this is the first report on the synthesis of bioactive metal nanoparticles from and it may open up new avenues in therapeutic applications.

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References

Baharara J, Namvar F, Ramezani T, Mousavi M, Mohamad R . Silver nanoparticles biosynthesized using Achillea biebersteinii flower extract: apoptosis induction in MCF-7 cells via caspase activation and regulation of Bax and Bcl-2 gene expression. Molecules. 2015; 20(2):2693-706. PMC: 6272258. DOI: 10.3390/molecules20022693. View

Kumar C, Poornachandra Y, Chandrasekhar C . Green synthesis of bacterial mediated anti-proliferative gold nanoparticles: inducing mitotic arrest (G2/M phase) and apoptosis (intrinsic pathway). Nanoscale. 2015; 7(44):18738-50. DOI: 10.1039/c5nr04577k. View

Shibue T, Weinberg R . EMT, CSCs, and drug resistance: the mechanistic link and clinical implications. Nat Rev Clin Oncol. 2017; 14(10):611-629. PMC: 5720366. DOI: 10.1038/nrclinonc.2017.44. View

Iravani S, Korbekandi H, Mirmohammadi S, Zolfaghari B . Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2015; 9(6):385-406. PMC: 4326978. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

Muramatsu Y, Maemoto T, Iwashita A, Matsuoka N . Novel neuroprotective compound SCH-20148 rescues thymocytes and SH-SY5Y cells from thapsigargin-induced mitochondrial membrane potential reduction and cell death. Eur J Pharmacol. 2007; 563(1-3):40-8. DOI: 10.1016/j.ejphar.2007.01.060. View

Kumar C, Poornachandra Y . Biodirected synthesis of Miconazole-conjugated bacterial silver nanoparticles and their application as antifungal agents and drug delivery vehicles. Colloids Surf B Biointerfaces. 2014; 125:110-9. DOI: 10.1016/j.colsurfb.2014.11.025. View

Lu L, Sun R, Chen R, Hui C, Ho C, Luk J . Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther. 2008; 13(2):253-62. View

Wang Q, Zhang L, Yuan X, Ou Y, Zhu X, Cheng Z . The Relationship between the Bcl-2/Bax Proteins and the Mitochondria-Mediated Apoptosis Pathway in the Differentiation of Adipose-Derived Stromal Cells into Neurons. PLoS One. 2016; 11(10):e0163327. PMC: 5051896. DOI: 10.1371/journal.pone.0163327. View

10.

Huang H, Yang X . Synthesis of polysaccharide-stabilized gold and silver nanoparticles: a green method. Carbohydr Res. 2004; 339(15):2627-31. DOI: 10.1016/j.carres.2004.08.005. View

11.

Vasanth K, Ilango K, Mohankumar R, Agrawal A, Dubey G . Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids Surf B Biointerfaces. 2014; 117:354-9. DOI: 10.1016/j.colsurfb.2014.02.052. View

12.

Couvreur P, Vauthier C . Nanotechnology: intelligent design to treat complex disease. Pharm Res. 2006; 23(7):1417-50. DOI: 10.1007/s11095-006-0284-8. View

13.

Hinterwirth H, Kappel S, Waitz T, Prohaska T, Lindner W, Lammerhofer M . Quantifying thiol ligand density of self-assembled monolayers on gold nanoparticles by inductively coupled plasma-mass spectrometry. ACS Nano. 2013; 7(2):1129-36. PMC: 3584655. DOI: 10.1021/nn306024a. View

14.

Sharma N, Sahi S, Nath S, Parsons J, Gardea-Torresdey J, Pal T . Synthesis of plant-mediated gold nanoparticles and catalytic role of biomatrix-embedded nanomaterials. Environ Sci Technol. 2007; 41(14):5137-42. PMC: 2518977. DOI: 10.1021/es062929a. View

15.

Jayachandra Reddy N, Nagoor Vali D, Rani M, Sudha Rani S . Evaluation of antioxidant, antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit. Mater Sci Eng C Mater Biol Appl. 2013; 34:115-22. DOI: 10.1016/j.msec.2013.08.039. View

16.

Shankar S, Rai A, Ahmad A, Sastry M . Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004; 275(2):496-502. DOI: 10.1016/j.jcis.2004.03.003. View

17.

Castro-Aceituno V, Abbai R, Moon S, Ahn S, Mathiyalagan R, Kim Y . Pleuropterus multiflorus (Hasuo) mediated straightforward eco-friendly synthesis of silver, gold nanoparticles and evaluation of their anti-cancer activity on A549 lung cancer cell line. Biomed Pharmacother. 2017; 93:995-1003. DOI: 10.1016/j.biopha.2017.07.040. View

18.

Poojary M, Vishnumurthy K, Vasudeva Adhikari A . Extraction, characterization and biological studies of phytochemicals from . J Pharm Anal. 2018; 5(3):182-189. PMC: 5762209. DOI: 10.1016/j.jpha.2015.01.002. View

19.

Soderstjerna E, Bauer P, Cedervall T, Abdshill H, Johansson F, Englund Johansson U . Silver and gold nanoparticles exposure to in vitro cultured retina--studies on nanoparticle internalization, apoptosis, oxidative stress, glial- and microglial activity. PLoS One. 2014; 9(8):e105359. PMC: 4140780. DOI: 10.1371/journal.pone.0105359. View

20.

Fazal H, Abbasi B, Ahmad N, Ali M . Elicitation of Medicinally Important Antioxidant Secondary Metabolites with Silver and Gold Nanoparticles in Callus Cultures of Prunella vulgaris L. Appl Biochem Biotechnol. 2016; 180(6):1076-1092. DOI: 10.1007/s12010-016-2153-1. View