: Public Health Burden Associated with Ready-to-eat Foods in Himachal Pradesh, India

Overview

Journal J Food Sci Technol

Specialty Nutritional Sciences

Date 2020 May 21

PMID 32431355

Citations 8

Authors

Neha Rana

Ashok Kumar Panda

Nina Pathak

Tania Gupta

Sidharath Dev Thakur

Affiliations

Soon will be listed here.

Abstract

The study determined incidence, enterotoxigenecity and antimicrobial susceptibility profiles of isolated from ready-to-eat (RTE) milk products (n = 80), RTE meat products (n = 40), beverages (n = 40) and water samples (n = 60, from food preparing and serving outlets/restaurants) collected from eight different tourist places of Himachal Pradesh. 11.4% (25/220) samples were contaminated with and isolates were identified as (76.0%, n = 19), (12.0%, n = 3) (8.0%, n = 2) and (4.0%, n = 1) by conventional and molecular methods. incidence was highest in cheese based foods (25.0%) followed by vegetable soups (16.7%), khoa based foods (14.0%), milk based beverages (10.5%), paneer based foods (8.6%), cream based foods (8.3%) and water (8.3%) samples. Multiplex polymerase chain reaction detected enterotoxigenic genes only in isolates. complex (encoding non-haemolytic enterotoxins, ABC) genes were detected only in isolates. 57.6% (11/19), 36.8% (7/19) and 5.3% (1/19) harboured all three (, ), two () and one () gene, respectively. Among complex genes (encoding haemolytic enterotoxins CAD), only (36.8%, 7/19) was detected. Incidence (encoding cytotoxin enterotoxin) was 52.6% (10/19). Each isolate harboured two or more enterotoxigenic genes. Seven isolates had at least one gene from haemolytic and non-haemolytic complexes along with . High levels (> 50%) of antimicrobial resistance were recorded for penicillin, amoxicillin, ampicillin cefixime and ceftazidine in tested isolates. Two isolates were identified as multidrug resistant isolates with resistance to ≥ 3 antibiotic classes.

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References

Andersson A, Ronner U, Granum P . What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens?. Int J Food Microbiol. 1995; 28(2):145-55. DOI: 10.1016/0168-1605(95)00053-4. View

Bottone E . Bacillus cereus, a volatile human pathogen. Clin Microbiol Rev. 2010; 23(2):382-98. PMC: 2863360. DOI: 10.1128/CMR.00073-09. View

Lakhanpal P, Panda A, Chahota R, Choudhary S, Thakur S . Incidence and antimicrobial susceptibility of isolated from ready-to-eat foods of animal origin from tourist destinations of North-western Himalayas, Himachal Pradesh, India. J Food Sci Technol. 2019; 56(2):1078-1083. PMC: 6400743. DOI: 10.1007/s13197-018-03556-x. View

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

Kotiranta A, Lounatmaa K, Haapasalo M . Epidemiology and pathogenesis of Bacillus cereus infections. Microbes Infect. 2000; 2(2):189-98. DOI: 10.1016/s1286-4579(00)00269-0. View

Yusuf U, Kotwal S, Gupta S, Ahmed T . Identification and antibiogram pattern of from the milk and milk products in and around Jammu region. Vet World. 2018; 11(2):186-191. PMC: 5891873. DOI: 10.14202/vetworld.2018.186-191. View

Gopal N, Hill C, Ross P, Beresford T, Fenelon M, Cotter P . The Prevalence and Control of Bacillus and Related Spore-Forming Bacteria in the Dairy Industry. Front Microbiol. 2016; 6:1418. PMC: 4685140. DOI: 10.3389/fmicb.2015.01418. View

Berthold-Pluta A, Pluta A, Garbowska M . The effect of selected factors on the survival of Bacillus cereus in the human gastrointestinal tract. Microb Pathog. 2015; 82:7-14. DOI: 10.1016/j.micpath.2015.03.015. View

Tewari A, Abdullah S . Bacillus cereus food poisoning: international and Indian perspective. J Food Sci Technol. 2015; 52(5):2500-11. PMC: 4397285. DOI: 10.1007/s13197-014-1344-4. View

10.

Kirk M, Pires S, Black R, Caipo M, Crump J, Devleesschauwer B . World Health Organization Estimates of the Global and Regional Disease Burden of 22 Foodborne Bacterial, Protozoal, and Viral Diseases, 2010: A Data Synthesis. PLoS Med. 2015; 12(12):e1001921. PMC: 4668831. DOI: 10.1371/journal.pmed.1001921. View

11.

Tirloni E, Ghelardi E, Celandroni F, Bernardi C, Stella S . Effect of dairy product environment on the growth of Bacillus cereus. J Dairy Sci. 2017; 100(9):7026-7034. DOI: 10.3168/jds.2017-12978. View

12.

Beecher D, Schoeni J, Wong A . Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect Immun. 1995; 63(11):4423-8. PMC: 173629. DOI: 10.1128/iai.63.11.4423-4428.1995. View

13.

Owusu-Kwarteng J, Wuni A, Akabanda F, Tano-Debrah K, Jespersen L . Prevalence, virulence factor genes and antibiotic resistance of Bacillus cereus sensu lato isolated from dairy farms and traditional dairy products. BMC Microbiol. 2017; 17(1):65. PMC: 5348786. DOI: 10.1186/s12866-017-0975-9. View

14.

Al-Khatib M, Khyami-Horani H, Badran E, Shehabi A . Incidence and characterization of diarrheal enterotoxins of fecal Bacillus cereus isolates associated with diarrhea. Diagn Microbiol Infect Dis. 2007; 59(4):383-7. DOI: 10.1016/j.diagmicrobio.2007.06.014. View

15.

Tewari A, Singh S, Singh R . Incidence and enterotoxigenic profile of Bacillus cereus in meat and meat products of Uttarakhand, India. J Food Sci Technol. 2015; 52(3):1796-801. PMC: 4348265. DOI: 10.1007/s13197-013-1162-0. View

16.

Fernandez-No I, Guarddon M, Bohme K, Cepeda A, Calo-Mata P, Barros-Velazquez J . Detection and quantification of spoilage and pathogenic Bacillus cereus, Bacillus subtilis and Bacillus licheniformis by real-time PCR. Food Microbiol. 2011; 28(3):605-10. DOI: 10.1016/j.fm.2010.10.014. View

17.

Yang I, Shih D, Huang T, Huang Y, Wang J, Pan T . Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. J Food Prot. 2005; 68(10):2123-30. DOI: 10.4315/0362-028x-68.10.2123. View

18.

Ngamwongsatit P, Buasri W, Pianariyanon P, Pulsrikarn C, Ohba M, Assavanig A . Broad distribution of enterotoxin genes (hblCDA, nheABC, cytK, and entFM) among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int J Food Microbiol. 2007; 121(3):352-6. DOI: 10.1016/j.ijfoodmicro.2007.11.013. View

19.

Keisam S, Tuikhar N, Ahmed G, Jeyaram K . Toxigenic and pathogenic potential of enteric bacterial pathogens prevalent in the traditional fermented foods marketed in the Northeast region of India. Int J Food Microbiol. 2019; 296:21-30. DOI: 10.1016/j.ijfoodmicro.2019.02.012. View

20.

Logan N . Bacillus and relatives in foodborne illness. J Appl Microbiol. 2011; 112(3):417-29. DOI: 10.1111/j.1365-2672.2011.05204.x. View