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Influence of Storage Conditions on SARS-CoV-2 Nucleic Acid Detection in Throat Swabs

Overview
Journal J Infect Dis
Publisher Oxford University Press
Specialty Infectious Diseases
Date 2020 May 20
PMID 32427340
Citations 27
Authors
Lin Li
Xiao Li
Zhendong Guo
Zhongyi Wang
Ke Zhang
Chao Li
Changjun Wang
Shoufeng Zhang
Affiliations
Soon will be listed here.
Abstract

The detection of SARS-CoV-2 infection is the premise of quarantine. In many countries or areas, samples need to be shipped or inactivated before SARS-CoV-2 testing. In this study, we checked the influence of sample storage conditions on SARS-CoV-2 nucleic acid testing results, including sample inactivation time, storage temperature, and storage time. All of these conditions caused an increase in the cycle threshold values of the nucleic acid tests and led to the misclassification of at least 10.2% of positive cases as negative or suspected. The results highlight the importance of immediate testing of samples for SARS-CoV-2 nucleic acid detection.

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References
1.
Zhu N, Zhang D, Wang W, Li X, Yang B, Song J . A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med. 2020; 382(8):727-733. PMC: 7092803. DOI: 10.1056/NEJMoa2001017. View
2.
Corman V, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu D . Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020; 25(3). PMC: 6988269. DOI: 10.2807/1560-7917.ES.2020.25.3.2000045. View
3.
Guo Z, Wang Z, Zhang S, Li X, Li L, Li C . Aerosol and Surface Distribution of Severe Acute Respiratory Syndrome Coronavirus 2 in Hospital Wards, Wuhan, China, 2020. Emerg Infect Dis. 2020; 26(7):1583-1591. PMC: 7323510. DOI: 10.3201/eid2607.200885. View