ST8SIA6-AS1 Promotes Hepatocellular Carcinoma by Absorbing MiR-5195-3p to Regulate HOXB6

Overview

Journal Cancer Biol Ther

Specialties Oncology
Pharmacology

Date 2020 May 19

PMID 32420798

Citations 23

Authors

Yang Li

An Jiang

Affiliations

Soon will be listed here.

Abstract

Background: Deemed as a member of malignant tumors, hepatocellular carcinoma (HCC) has been characterized as a lethal disease with high morbidity and mortality. It has been widely accepted that long noncoding RNAs (lncRNAs) play a big part in the complicated biologic processes of cancer.

Aim Of The Study: The purpose of the study is to figure out the role and molecular regulation mechanism of ST8SIA6-AS1 in HCC.

Methods: The role of ST8SIA6-AS1 in HCC was validated by RT-qPCR, colony formation, ki-67 detection, TUNEL, JC-1 detection, wound healing and transwell-invasion assays, furthermore, the binding ability between ST8SIA6-AS1/HOXB6 and miR-5195-3p were confirmed by RNA pull down and luciferase reporter assays. Besides, the regulatory mechanism of ST8SIA6-AS1 to HOXB6/miR-5195-3p was measured by RT-qPCR and western blot assays.

Results: We measured that ST8SIA6-AS1 was highly expressed in HCC cell lines. Then knockdown of it suppressed cell proliferation, migration and migration but activated cell apoptosis in HCC. Furthermore, ST8SIA6-AS1 could bind with miR-5195-3p and negatively regulated its expression in HCC. Subsequently, it confirmed that HOXB6 was target gene of miR-5195-3p and positively modulated by ST8SIA6-AS1 in HCC. Finally, we verified that miR-5195-3p deficiency or HOXB6 upregulation countervailed the repressing effects of ST8SIA6-AS1 depletion on HCC progression.

Conclusions: To sum up, ST8SIA6-AS1 promotes HCC progression by absorbing miR-5195-3p to regulate HOXB6, which might provide some worthy suggestions to research the development process of HCC.

Citing Articles

Circulating tumor DNA methylation markers for the early diagnosis of hepatocellular carcinoma.

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Crispr-Cas9-based long non-coding RNA interference and activation identified that the aberrant expression of Myc-regulated ST8SIA6 antisense RNA 1 promotes tumorigenesis and metastasis in hepatocellular carcinoma.

Liu X, Jiang D, Liu Y, Xie K, Zhao Y, Liu F Cytojournal. 2024; 21:53.

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MiR-5195-3p predicts clinical prognosis and represses colorectal cancer progression by targeting TLR4/MyD88 signaling.

Lv Y, Guo S, Jin L, Wang K, Li Y, Li H Cell Div. 2024; 19(1):29.

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ST8SIA6-AS1, a novel lncRNA star in liver cancer.

Qiu C, Fan H, Tao S, Deng Z, Luo H, Liu F Front Cell Dev Biol. 2024; 12:1435664.

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