Efficacy Evaluation of Anthelmintic Products Against an Infection with the Canine Hookworm (Ancylostoma Caninum) Isolate Worthy 4.1F3P in Dogs

Overview

Journal Int J Parasitol Drugs Drug Resist

Publisher Elsevier

Specialty Parasitology

Date 2020 May 14

PMID 32403053

Citations 12

Authors

Pablo D Jimenez Castro

Abdelmoneim Mansour

Samuel Charles

Joe Hostetler

Terry Settje

Daniel Kulke

Ray M Kaplan

Affiliations

Soon will be listed here.

Abstract

Ancylostoma caninum is the most prevalent intestinal nematode of dogs, and has a zoonotic potential. Multiple-drug resistance (MDR) has been confirmed in a number of A. caninum isolates, including isolate Worthy 4.1F3P, against all anthelmintic drug classes approved for hookworm treatment in dogs in the United States (US). The cyclooctadepsipeptide emodepside is not registered to use in dogs in the US, but in a number of other countries/regions. The objective of this study was to evaluate the efficacy of emodepside + praziquantel, as well as three commercial products that are commonly used in the US for treatment of hookworms, against a suspected (subsequently confirmed) MDR A. caninum isolate Worthy 4.1F3P. 40 dogs infected on study day (SD) 0 with 300 third-stage larvae, were randomly allocated to one of five treatment groups with eight dogs each: pyrantel pamoate (Nemex®-2), fenbendazole (Panacur® C), milbemycin oxime (Interceptor®), emodepside + praziquantel tablets and non-treated control. Fecal egg counts (FEC) were performed on SDs 19, 20, 22, 27, 31 and 34. All treatments were administered as per label requirements on SD 24 to dogs in Groups 1 through 4. Two additional treatments were administered on SDs 25 and 26 to dogs in Group 2 as per label requirements. Dogs were necropsied on SD 34 and the digestive tract was removed/processed for worm recovery and enumeration. The geometric mean (GM) worm counts for the control group was 97.4, and for the pyrantel pamoate, fenbendazole, milbemycin oxime, and emodepside + praziquantel groups were 74.8, 72.0, 88.9, and 0.4, respectively. These yielded efficacies of 23.2%, 26.1%, and 8.8%, and 99.6%, respectively. These data support previous findings of the MDR status of Worthy 4.1F3P as treatments with pyrantel pamoate, fenbendazole and milbemycin oxime lacked efficacy. In sharp contrast, Worthy 4.1F3P was highly susceptible to treatment with emodepside + praziquantel.

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References

Kopp S, Coleman G, McCarthy J, Kotze A . Phenotypic characterization of two Ancylostoma caninum isolates with different susceptibilities to the anthelmintic pyrantel. Antimicrob Agents Chemother. 2008; 52(11):3980-6. PMC: 2573120. DOI: 10.1128/AAC.00523-08. View

Kulke D, von Samson-Himmelstjerna G, Miltsch S, Wolstenholme A, Jex A, Gasser R . Characterization of the Ca2+-gated and voltage-dependent K+-channel Slo-1 of nematodes and its interaction with emodepside. PLoS Negl Trop Dis. 2014; 8(12):e3401. PMC: 4270693. DOI: 10.1371/journal.pntd.0003401. View

Kalkofen U . Hookworms of dogs and cats. Vet Clin North Am Small Anim Pract. 1987; 17(6):1341-54. DOI: 10.1016/s0195-5616(87)50005-5. View

Zahner H, Schares G . Experimental chemotherapy of filariasis: comparative evaluation of the efficacy of filaricidal compounds in Mastomys coucha infected with Litomosoides carinii, Acanthocheilonema viteae, Brugia malayi and B. pahangi. Acta Trop. 1993; 52(4):221-66. DOI: 10.1016/0001-706x(93)90010-9. View

Kaplan R, Vidyashankar A . An inconvenient truth: global worming and anthelmintic resistance. Vet Parasitol. 2011; 186(1-2):70-8. DOI: 10.1016/j.vetpar.2011.11.048. View

Sasaki T, Takagi M, Yaguchi T, Miyadoh S, Okada T, Koyama M . A new anthelmintic cyclodepsipeptide, PF1022A. J Antibiot (Tokyo). 1992; 45(5):692-7. DOI: 10.7164/antibiotics.45.692. View

Hess L, Millward L, Rudinsky A, Vincent E, Marsh A . Combination Anthelmintic Treatment for Persistent Ova Shedding in Greyhounds. J Am Anim Hosp Assoc. 2019; 55(3):160-166. DOI: 10.5326/JAAHA-MS-6904. View

Macrelli M, Williamson S, Mitchell S, Pearson R, Andrews L, Morrison A . First detection of ivermectin resistance in oesophagostomum dentatum in pigs. Vet Parasitol. 2019; 270:1-6. DOI: 10.1016/j.vetpar.2019.05.002. View

Welz C, Kruger N, Schniederjans M, Miltsch S, Krucken J, Guest M . SLO-1-channels of parasitic nematodes reconstitute locomotor behaviour and emodepside sensitivity in Caenorhabditis elegans slo-1 loss of function mutants. PLoS Pathog. 2011; 7(4):e1001330. PMC: 3072372. DOI: 10.1371/journal.ppat.1001330. View

10.

Kruger N, Harder A, von Samson-Himmelstjerna G . The putative cyclooctadepsipeptide receptor depsiphilin of the canine hookworm Ancylostoma caninum. Parasitol Res. 2009; 105 Suppl 1:S91-100. DOI: 10.1007/s00436-009-1500-3. View

11.

Kachi S, Terada M, Hashimoto H . Effects of amorphous and polymorphs of PF1022A, a new antinematode drug, on Angiostrongylus costaricensis in mice. Jpn J Pharmacol. 1998; 77(3):235-45. DOI: 10.1254/jjp.77.235. View

12.

Harder A, Schmitt-Wrede H, Krucken J, Marinovski P, Wunderlich F, Willson J . Cyclooctadepsipeptides--an anthelmintically active class of compounds exhibiting a novel mode of action. Int J Antimicrob Agents. 2003; 22(3):318-31. DOI: 10.1016/s0924-8579(03)00219-x. View

13.

Drake J, Carey T . Seasonality and changing prevalence of common canine gastrointestinal nematodes in the USA. Parasit Vectors. 2019; 12(1):430. PMC: 6728981. DOI: 10.1186/s13071-019-3701-7. View

14.

Guest M, Bull K, Walker R, Amliwala K, OConnor V, Harder A . The calcium-activated potassium channel, SLO-1, is required for the action of the novel cyclo-octadepsipeptide anthelmintic, emodepside, in Caenorhabditis elegans. Int J Parasitol. 2007; 37(14):1577-88. DOI: 10.1016/j.ijpara.2007.05.006. View

15.

Kopp S, Coleman G, McCarthy J, Kotze A . Application of in vitro anthelmintic sensitivity assays to canine parasitology: detecting resistance to pyrantel in Ancylostoma caninum. Vet Parasitol. 2008; 152(3-4):284-93. DOI: 10.1016/j.vetpar.2007.12.020. View

16.

Bowman D, Montgomery S, Zajac A, Eberhard M, Kazacos K . Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitol. 2010; 26(4):162-7. DOI: 10.1016/j.pt.2010.01.005. View

17.

Del Giudice P, Hakimi S, Vandenbos F, Magana C, Hubiche T . Autochthonous Cutaneous Larva Migrans in France and Europe. Acta Derm Venereol. 2019; 99(9):805-808. DOI: 10.2340/00015555-3217. View

18.

LEEMING J . Cutaneous larva migrans. S Afr Med J. 1966; 40(18):403-5. View

19.

von Samson-Himmelstjerna G, Harder A, Sangster N, Coles G . Efficacy of two cyclooctadepsipeptides, PF1022A and emodepside, against anthelmintic-resistant nematodes in sheep and cattle. Parasitology. 2005; 130(Pt 3):343-7. DOI: 10.1017/s0031182004006523. View

20.

Chen W, Terada M, Cheng J . Characterization of subtypes of gamma-aminobutyric acid receptors in an Ascaris muscle preparation by binding assay and binding of PF1022A, a new anthelmintic, on the receptors. Parasitol Res. 1996; 82(2):97-101. DOI: 10.1007/s004360050077. View