Eckol from Suppresses Immunoglobulin E-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mice

Overview

Journal Nutrients

Date 2020 May 14

PMID 32397556

Citations 8

Authors

Eui Jeong Han

Hyun-Soo Kim

K K A Sanjeewa

K H I N M Herath

You-Jin Jeon

Youngheun Jee

Jeongjun Lee

Taehee Kim

Sun-Yup Shim

Ginnae Ahn

Affiliations

Soon will be listed here.

Abstract

Eckol, a precursor compound belonging to the dibenzo-1,4-dioxin class of phlorotannins, is a phloroglucinol derivative that exerts various activities. In the present study, we investigated the antiallergic effects of eckol isolated from the marine brown algae, using immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated mouse bone marrow-derived cultured mast cells (BMCMC) and a mouse model of anaphylaxis. Eckol inhibited IgE/BSA-induced BMCMC degranulation by reducing β-hexosaminidase release. A flow cytometric analysis revealed that eckol decreases FcεRI expression on cell surface and IgE binding to the FcεRI in BMCMC. Moreover, eckol suppressed the production of the cytokines, interleukin (IL)-4, IL-5, IL-6, and IL-13 and the chemokine, thymus activation-regulated chemokine (TARC) by downregulating, IκB-α degradation and NF-κB nuclear translocation. Furthermore, it attenuated the passive cutaneous anaphylactic reaction induced by IgE/BSA-stimulation in the ear of BALB/c mice. These results suggest that eckol is a potential therapeutic candidate for the prevention and treatment of allergic disorders.

Citing Articles

Marine Pharmacology in 2019-2021: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous....

Mayer A, Mayer V, Swanson-Mungerson M, Pierce M, Rodriguez A, Nakamura F Mar Drugs. 2024; 22(7).

PMID: 39057418 PMC: 11278370. DOI: 10.3390/md22070309.

Protective Effect and Mechanisms of Eckol on Chronic Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice.

Liao M, Wei S, Hu X, Liu J, Wang J Mar Drugs. 2023; 21(7).

PMID: 37504907 PMC: 10381161. DOI: 10.3390/md21070376.

Structures and Anti-Allergic Activities of Natural Products from Marine Organisms.

Chen N, Zhang S, Javeed A, Jian C, Liu Y, Sun J Mar Drugs. 2023; 21(3).

PMID: 36976202 PMC: 10056057. DOI: 10.3390/md21030152.

Plant-Derived Molecule 4-Methylumbelliferone Suppresses FcεRI-Mediated Mast Cell Activation and Allergic Inflammation.

Wang H, Xiang Q, Lin H, Chen J, Guo W, Guo W Molecules. 2022; 27(5).

PMID: 35268679 PMC: 8912031. DOI: 10.3390/molecules27051577.

Anti-Allergic Effect of 3,4-Dihydroxybenzaldehyde Isolated from in IgE/BSA-Stimulated Mast Cells and a Passive Cutaneous Anaphylaxis Mouse Model.

Kim E, Han E, Kim J, Fernando I, Oh J, Kim K Mar Drugs. 2022; 20(2).

PMID: 35200662 PMC: 8875385. DOI: 10.3390/md20020133.

References

Jun Y, Lee M, Shin T, Yoon N, Kim J, Kim H . eckol enhances heme oxygenase-1 expression through activation of Nrf2/JNK pathway in HepG2 cells. Molecules. 2014; 19(10):15638-52. PMC: 6271008. DOI: 10.3390/molecules191015638. View

Isaza Martinez J, Torres Castaneda H . Preparation and chromatographic analysis of phlorotannins. J Chromatogr Sci. 2013; 51(8):825-38. DOI: 10.1093/chromsci/bmt045. View

Li Y, Lee S, Le Q, Kim M, Kim S . Anti-allergic effects of phlorotannins on histamine release via binding inhibition between IgE and Fc epsilonRI. J Agric Food Chem. 2008; 56(24):12073-80. DOI: 10.1021/jf802732n. View

Arshad S . Does exposure to indoor allergens contribute to the development of asthma and allergy?. Curr Allergy Asthma Rep. 2010; 10(1):49-55. DOI: 10.1007/s11882-009-0082-6. View

Johnson D, Douglas I, Jahnke A, Ghosh S, Pober J . A sustained reduction in IkappaB-beta may contribute to persistent NF-kappaB activation in human endothelial cells. J Biol Chem. 1996; 271(27):16317-22. DOI: 10.1074/jbc.271.27.16317. View

Manandhar B, Paudel P, Seong S, Jung H, Choi J . Characterizing Eckol as a Therapeutic Aid: A Systematic Review. Mar Drugs. 2019; 17(6). PMC: 6627842. DOI: 10.3390/md17060361. View

Mukai K, Tsai M, Saito H, Galli S . Mast cells as sources of cytokines, chemokines, and growth factors. Immunol Rev. 2018; 282(1):121-150. PMC: 5813811. DOI: 10.1111/imr.12634. View

Hakimi J, Seals C, Kondas J, Pettine L, Danho W, Kochan J . The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J Biol Chem. 1990; 265(36):22079-81. View

Lee S, Youn K, Kim D, Ahn M, Yoon E, Kim O . Anti-Neuroinflammatory Property of Phlorotannins from on Aβ-Induced Damage in PC12 Cells. Mar Drugs. 2018; 17(1). PMC: 6356621. DOI: 10.3390/md17010007. View

10.

Tkaczyk C, Frandji P, Botros H, Poncet P, LAPEYRE J, Peronet R . Mouse bone marrow-derived mast cells and mast cell lines constitutively produce B cell growth and differentiation activities. J Immunol. 1996; 157(4):1720-8. View

11.

Kinet J, Blank U, BRINI A, Jouvin M, Kuster H, Mejan O . The high-affinity receptor for immunoglobulin E: a target for therapy of allergic diseases. Int Arch Allergy Appl Immunol. 1991; 94(1-4):51-5. DOI: 10.1159/000235323. View

12.

Loureiro C, Medema M, van der Oost J, Sipkema D . Exploration and exploitation of the environment for novel specialized metabolites. Curr Opin Biotechnol. 2018; 50:206-213. DOI: 10.1016/j.copbio.2018.01.017. View

13.

Kang M, Cha S, Wijesinghe W, Kang S, Lee S, Kim E . Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo. Food Chem. 2013; 138(2-3):950-5. DOI: 10.1016/j.foodchem.2012.11.005. View

14.

Kang H, Young Chung H, Kim J, Son B, Jung H, Choi J . Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch Pharm Res. 2004; 27(2):194-8. DOI: 10.1007/BF02980106. View

15.

Kim S, Kang K, Jeon J, Jho E, Kim C, Nho C . Isolation of phlorotannins from Eisenia bicyclis and their hepatoprotective effect against oxidative stress induced by tert-butyl hyperoxide. Appl Biochem Biotechnol. 2011; 165(5-6):1296-307. DOI: 10.1007/s12010-011-9347-3. View

16.

Zhang M, Guo J, Hu X, Zhao S, Li S, Wang J . An in vivo anti-tumor effect of eckol from marine brown algae by improving the immune response. Food Funct. 2019; 10(7):4361-4371. DOI: 10.1039/c9fo00865a. View

17.

Galli S, Gordon J, Wershil B . Cytokine production by mast cells and basophils. Curr Opin Immunol. 1991; 3(6):865-72. DOI: 10.1016/s0952-7915(05)80005-6. View

18.

Do H, Hwang Y, Yang H, Park K . Effect of Extract on IgE-Antigen-Mediated Allergic Reaction in Rat Basophilic Leukemic RBL-2H3 Mast Cells and Passive Cutaneous Anaphylaxis in Mice. Evid Based Complement Alternat Med. 2019; 2019:6497691. PMC: 6811800. DOI: 10.1155/2019/6497691. View

19.

Kiuru P, DAuria M, Muller C, Tammela P, Vuorela H, Yli-Kauhaluoma J . Exploring marine resources for bioactive compounds. Planta Med. 2014; 80(14):1234-46. DOI: 10.1055/s-0034-1383001. View

20.

Kim A, Shin T, Lee M, Park J, Park K, Yoon N . Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J Agric Food Chem. 2009; 57(9):3483-9. DOI: 10.1021/jf900820x. View