Multiplex Precise Base Editing in Cynomolgus Monkeys

Overview

Journal Nat Commun

Specialty Biology

Date 2020 May 13

PMID 32393762

Citations 26

Authors

Wenhui Zhang

Tomomi Aida

Ricardo C H Del Rosario

Jonathan J Wilde

Chenhui Ding

Xiaohui Zhang

Zulqurain Baloch

Yan Huang

Yu Tang

Duanduan Li

Hongyu Lu

Yang Zhou

Minqing Jiang

Dongdong Xu

Zhihao Fang

Zhanhong Zheng

Qunshan Huang

Guoping Feng

Shihua Yang

Affiliations

Soon will be listed here.

Abstract

Common polygenic diseases result from compounded risk contributed by multiple genetic variants, meaning that simultaneous correction or introduction of single nucleotide variants is required for disease modeling and gene therapy. Here, we show precise, efficient, and simultaneous multiplex base editing of up to three target sites across 11 genes/loci in cynomolgus monkey embryos using CRISPR-based cytidine- and adenine-base editors. Unbiased whole genome sequencing demonstrates high specificity of base editing in monkey embryos. Our data demonstrate feasibility of multiplex base editing for polygenic disease modeling in primate zygotes.

Citing Articles

Pancreatic agenesis and altered m6A methylation in the pancreas of PDX1-mutant cynomolgus macaques.

Zhang W, Zhuang J, Guo Y, Chen X, Li Y, Xu J Zool Res. 2024; 45(6):1188-1200.

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Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.

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Deconstructing cancer with precision genome editing.

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Embryo and fetal gene editing: Technical challenges and progress toward clinical applications.

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Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation.

Wang Y, Chen J, Huang X, Wu B, Dai P, Zhang F Sci China Life Sci. 2024; 67(5):1035-1050.

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