Monocyte Apoptotic Bodies Are Vehicles for Influenza A Virus Propagation

Overview

Journal Commun Biol

Specialty Biology

Date 2020 May 10

PMID 32385344

Citations 17

Authors

Georgia K Atkin-Smith

Mubing Duan

Damien J Zanker

Liyen Loh

Thi H O Nguyen

Marios Koutsakos

Tien Nguyen

Xiangrui Jiang

Julio Carrera

Thanh Kha Phan

Chuanxin Liu

Stephanie Paone

Sara Oveissi

Amy L Hodge

Amy A Baxter

Katherine Kedzierska

Jason M Mackenzie

Mark D Hulett

Pamuk Bilsel

Weisan Chen

Ivan K H Poon

Affiliations

Soon will be listed here.

Abstract

The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functional significance of this process is not well defined. We recently discovered a new membrane protrusion (termed beaded apoptopodia) generated by apoptotic monocytes which fragments to release an abundance of ApoBDs. To investigate the function of apoptotic monocyte disassembly, we used influenza A virus (IAV) infection as a proof-of-concept model, as IAV commonly infects monocytes in physiological settings. We show that ApoBDs generated from IAV-infected monocytes contained IAV mRNA, protein and virions and consequently, could facilitate viral propagation in vitro and in vivo, and induce a robust antiviral immune response. We also identified an antipsychotic, Haloperidol, as an unexpected inhibitor of monocyte cell disassembly which could impair ApoBD-mediated viral propagation under in vitro conditions. Together, this study reveals a previously unrecognised function of apoptotic monocyte disassembly in the pathogenesis of IAV infections.

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