High Primary Antibiotic Resistance of Strains Isolated from Pediatric and Adult Patients in Poland During 2016-2018

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2020 May 7

PMID 32370201

Citations 10

Authors

Pawel Krzyzek

Dorota Pawelka

Barbara Iwanczak

Radoslaw Kempinski

Konrad Lesniakowski

Francis Megraud

Lukasz Laczmanski

Monika Biernat

Grazyna Gosciniak

Affiliations

Soon will be listed here.

Abstract

Monitoring the antibiotic resistance of is an important step in the effective treatment of this bacterium, thus the aim of the present study was to assess the prevalence of antimicrobial resistance of strains isolated from pediatric and adult patients with primary infections in 2016-2018. Antral biopsies from 334 treatment-naïve patients (126 children and 208 adults) were obtained. A total of 71 clinical strains (22 from children and 49 from adults) were isolated and examined for amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), tetracycline (TET), and levofloxacin (LEV) susceptibility. The activity of the antibiotics was measured by E-tests. Strains were considered as resistant to antibiotics with minimum inhibitory concentrations (MICs) equal to ≥0.125 μg/mL (AMX), ≥0.5 μg/mL (CLR), ≥8 μg/mL (MTZ), and ≥1 μg/mL (TET and LEV). The highest prevalence of antibiotic resistance in strains was observed for CLR and MTZ, at frequencies of 54.5% and 31.8% vs. 30.6% and 46.9% for children and adults, respectively. A much lower frequency of isolation of resistant strains was demonstrated for LEV and TET, this being 9.1% and 4.5% vs. 18.4% and 4.1% for pediatric and adult patients, respectively. The presence of AMX-resistant strains was not observed. The strains isolated from Polish patients with primary infections showed a high level of antibiotic resistance to CLR and MTZ (>30%).

Citing Articles

Global prevalence of Helicobacter pylori antibiotic resistance among children in the world health organization regions between 2000 and 2023: a systematic review and meta-analysis.

Salahi-Niri A, Nabavi-Rad A, Monaghan T, Rokkas T, Doulberis M, Sadeghi A BMC Med. 2024; 22(1):598.

PMID: 39710669 PMC: 11664859. DOI: 10.1186/s12916-024-03816-y.

Infections in Children.

Nguyen J, Kotilea K, Bontems P, Miendje Deyi V Antibiotics (Basel). 2023; 12(9).

PMID: 37760736 PMC: 10525885. DOI: 10.3390/antibiotics12091440.

Antibiotic-Resistant Strains of Helicobacter pylori in 50 Antibiotic Treatment-Naive Children in Northeast Poland Diagnosed by Gastric or Duodenal Biopsy Between February 2019 and May 2022.

Kucharska M, Zdanowicz K, Daniluk T, Bucki R, Lebensztejn D, Daniluk U Med Sci Monit. 2023; 29:e941195.

PMID: 37658600 PMC: 10481755. DOI: 10.12659/MSM.941195.

Current Worldwide Trends in Pediatric Antimicrobial Resistance.

Borka Balas R, Melit L, Marginean C Children (Basel). 2023; 10(2).

PMID: 36832532 PMC: 9954810. DOI: 10.3390/children10020403.

Antibiotic Resistance of in Children with Gastritis and Peptic Ulcers in Mekong Delta, Vietnam.

Le L, Nguyen T, Nguyen N, Nguyen Y, Nguyen H, Nguyen L Healthcare (Basel). 2022; 10(6).

PMID: 35742177 PMC: 9222858. DOI: 10.3390/healthcare10061121.

References

Guevara B, Cogdill A . Helicobacter pylori: A Review of Current Diagnostic and Management Strategies. Dig Dis Sci. 2020; 65(7):1917-1931. DOI: 10.1007/s10620-020-06193-7. View

Gosciniak G, Biernat M, Grabinska J, Binkowska A, Poniewierka E, Iwanczak B . The antimicrobial susceptibility of Helicobacter pylori strains isolated from children and adults with primary infection in the Lower Silesia Region, Poland. Pol J Microbiol. 2014; 63(1):57-61. View

Boyanova L, Ilieva J, Gergova G, Davidkov L, Spassova Z, Kamburov V . Numerous risk factors for Helicobacter pylori antibiotic resistance revealed by extended anamnesis: a Bulgarian study. J Med Microbiol. 2011; 61(Pt 1):85-93. DOI: 10.1099/jmm.0.035568-0. View

Shrestha P, Cooper B, Coast J, Oppong R, Do Thi Thuy N, Phodha T . Enumerating the economic cost of antimicrobial resistance per antibiotic consumed to inform the evaluation of interventions affecting their use. Antimicrob Resist Infect Control. 2018; 7:98. PMC: 6085682. DOI: 10.1186/s13756-018-0384-3. View

Venter H, Henningsen M, Begg S . Antimicrobial resistance in healthcare, agriculture and the environment: the biochemistry behind the headlines. Essays Biochem. 2017; 61(1):1-10. PMC: 5900547. DOI: 10.1042/EBC20160053. View

Megraud F . Helicobacter pylori and antibiotic resistance. Gut. 2007; 56(11):1502. PMC: 2095668. DOI: 10.1136/gut.2007.132514. View

Ducournau A, Benejat L, Sifre E, Bessede E, Lehours P, Megraud F . Helicobacter pylori resistance to antibiotics in 2014 in France detected by phenotypic and genotypic methods. Clin Microbiol Infect. 2016; 22(8):715-8. DOI: 10.1016/j.cmi.2016.06.003. View

Malfertheiner P, Megraud F, OMorain C, Gisbert J, Kuipers E, Axon A . Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report. Gut. 2016; 66(1):6-30. DOI: 10.1136/gutjnl-2016-312288. View

Lee J, Kim N, Nam R, Lee S, Soo In C, Kim J . Risk factors of rescue bismuth quadruple therapy failure for Helicobacter pylori eradication. J Gastroenterol Hepatol. 2019; 34(4):666-672. DOI: 10.1111/jgh.14625. View

10.

Nyssen O, Perez-Aisa A, Tepes B, Rodrigo-Saez L, Romero P, Lucendo A . Helicobacter pylori first-line and rescue treatments in patients allergic to penicillin: Experience from the European Registry on H pylori management (Hp-EuReg). Helicobacter. 2020; 25(3):e12686. DOI: 10.1111/hel.12686. View

11.

cizman M, Plankar Srovin T . Antibiotic consumption and resistance of gram-negative pathogens (collateral damage). GMS Infect Dis. 2019; 6:Doc05. PMC: 6301726. DOI: 10.3205/id000040. View

12.

Macias-Garcia F, Llovo-Taboada J, Diaz-Lopez M, Baston-Rey I, Dominguez-Munoz J . High primary antibiotic resistance of Helicobacter Pylori strains isolated from dyspeptic patients: A prevalence cross-sectional study in Spain. Helicobacter. 2017; 22(6). DOI: 10.1111/hel.12440. View

13.

Zhang W, Chen Q, Liang X, Liu W, Xiao S, Graham D . Bismuth, lansoprazole, amoxicillin and metronidazole or clarithromycin as first-line Helicobacter pylori therapy. Gut. 2015; 64(11):1715-20. DOI: 10.1136/gutjnl-2015-309900. View

14.

Saracino I, Fiorini G, Zullo A, Pavoni M, Saccomanno L, Vaira D . Trends in Primary Antibiotic Resistance in Strains Isolated in Italy between 2009 and 2019. Antibiotics (Basel). 2020; 9(1). PMC: 7168339. DOI: 10.3390/antibiotics9010026. View

15.

Savoldi A, Carrara E, Graham D, Conti M, Tacconelli E . Prevalence of Antibiotic Resistance in Helicobacter pylori: A Systematic Review and Meta-analysis in World Health Organization Regions. Gastroenterology. 2018; 155(5):1372-1382.e17. PMC: 6905086. DOI: 10.1053/j.gastro.2018.07.007. View

16.

Yu L, Luo L, Long X, Liang X, Ji Y, Graham D . High-dose PPI-amoxicillin dual therapy with or without bismuth for first-line Helicobacter pylori therapy: A randomized trial. Helicobacter. 2019; 24(4):e12596. DOI: 10.1111/hel.12596. View

17.

Klein E, Van Boeckel T, Martinez E, Pant S, Gandra S, Levin S . Global increase and geographic convergence in antibiotic consumption between 2000 and 2015. Proc Natl Acad Sci U S A. 2018; 115(15):E3463-E3470. PMC: 5899442. DOI: 10.1073/pnas.1717295115. View

18.

McMahon B, Hennessy T, Bensler J, Bruden D, Parkinson A, Morris J . The relationship among previous antimicrobial use, antimicrobial resistance, and treatment outcomes for Helicobacter pylori infections. Ann Intern Med. 2003; 139(6):463-9. DOI: 10.7326/0003-4819-139-6-200309160-00008. View

19.

Morilla A, Alvarez-Arguelles M, Duque J, Armesto E, Villar H, Melon S . Primary antimicrobial resistance rates and prevalence of Helicobacter pylori infection in the north of Spain. A 13-year retrospective study. Gastroenterol Hepatol. 2019; 42(8):476-485. DOI: 10.1016/j.gastrohep.2019.05.002. View

20.

Megraud F, Coenen S, Versporten A, Kist M, Lopez-Brea M, Hirschl A . Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut. 2012; 62(1):34-42. DOI: 10.1136/gutjnl-2012-302254. View