Chemically Synthesized Circular RNAs with Phosphoramidate Linkages Enable Rolling Circle Translation

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2020 May 6

PMID 32369063

Citations 6

Authors

Kosuke Nakamoto

Naoko Abe

Genichiro Tsuji

Yasuaki Kimura

Fumiaki Tomoike

Yoshihiro Shimizu

Hiroshi Abe

Affiliations

Soon will be listed here.

Abstract

Circular RNA without a stop codon enables rolling circle translation. To produce circular RNAs, we carried out one-pot chemical synthesis of circular RNA from RNA fragments with the use of an EDC/HOBt-based chemical ligation reaction. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.

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