ALDH1A1 Inhibits Chicken Preadipocytes' Proliferation and Differentiation Via the PPARγ Pathway In Vitro and In Vivo

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 May 6

PMID 32365706

Citations 9

Authors

Jing Zhang

Bolin Cai

Manting Ma

Wei Luo

Zipeng Zhang

Xiquan Zhang

Qinghua Nie

Affiliations

Soon will be listed here.

Abstract

(aldehyde dehydrogenase 1A1) is a crucial protein in retinoids' metabolism, and the lack of inhibits the fat deposition in mice. However, whether has a similar effect on chickens' fat-depot is still unknown. In this study, we investigate the role of in chickens' adipogenesis. The immortalized chicken preadipocyte 1 (ICP1) cell line and chicken primary preadipocytes isolated from abdominal fat were used to perform a series of experiments in vitro to elucidate the effects of . In addition, lentivirus was used to verify the results of cell experiments in vivo. The data showed that overexpression of significantly weakened the proliferation of preadipocytes and suppressed the differentiation of preadipocytes through the PPARγ pathway, and the knockdown experiments had the opposite results. Moreover, chickens injected with overexpression lentivirus had higher abdominal fat percentage, a bigger size of lipid droplets, and higher triglyceride content in abdominal fat, and chickens injected with interfering lentivirus had the opposite situation. We proved that not only inhibited the proliferation and differentiation of chickens' preadipocytes in vitro, but also inhibited the fat-depot of chickens in vivo, which was completely opposite the function of in mice, indicating that may have a different mechanism that is still unknown.

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