SKAP2 is Required for Defense Against Infection and Neutrophil Respiratory Burst

Overview

Journal Elife

Specialty Biology

Date 2020 May 1

PMID 32352382

Citations 15

Authors

Giang T Nguyen

Lamyaa Shaban

Matthias Mack

Kenneth D Swanson

Stephen C Bunnell

David B Sykes

Joan Mecsas

Affiliations

Soon will be listed here.

Abstract

is a respiratory, blood, liver, and bladder pathogen of significant clinical concern. We show that the adaptor protein, SKAP2, is required for protection against (ATCC 43816) pulmonary infections. - mice had 100-fold higher bacterial burden when compared to wild-type and burden was controlled by SKAP2 expression in innate immune cells. - neutrophils and monocytes were present in infected lungs, and the neutrophils degranulated normally in response to infection in mice; however, -stimulated reactive oxygen species (ROS) production in vitro was abolished. -induced neutrophil ROS response required the activity of SFKs, Syk, Btk, PLCγ2, and PKC. The loss of SKAP2 significantly hindered the -induced phosphorylation of SFKs, Syk, and Pyk2 implicating SKAP2 as proximal to their activation in pathogen-signaling pathways. In conclusion, SKAP2-dependent signaling in neutrophils is essential for -activated ROS production and for promoting bacterial clearance during infection.

Citing Articles

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SKAP2 acts downstream of CD11b/CD18 and regulates neutrophil effector function.

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