Circulating MicroRNAs As Biomarkers for Severe Coronary Artery Disease

Overview

Journal Medicine (Baltimore)

Specialty General Medicine

Date 2020 Apr 26

PMID 32332683

Citations 14

Authors

Xuelin Zhang

Haipeng Cai

Minqi Zhu

Yinfen Qian

Shanan Lin

Xiaoqiang Li

Affiliations

Soon will be listed here.

Abstract

Coronary artery disease (CAD) is the second leading cause of death after stroke in China. Percutaneous coronary intervention (PCI) significantly improves the prognosis of CAD patients. This study aimed to evaluate the diagnostic value of circulating microRNAs (miRNAs) in patients with severe CAD requiring PCI. The plasma miRNA profiles were determined using miRNA microarray. The relative expression levels of differentially expressed miRNA were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Nine miRNAs (ebv-miR-BART12, ebv-miR-BART16, let-7i-5p, miR-130a-3p, miR-26a-5p, miR-3149, miR-3152-3p, miR-32-3p, and miR-149-3p) were differentially expressed between severe CAD and control groups. Four miRNAs (let-7i-5p, miR-32-3p, miR-3149, and miR-26a-5p) validated by qRT-PCR showed good diagnostic accuracy, with the area under the receiver operating characteristic curves (AUCs) of 0.634 (95% confidence interval [CI] 0.528-0.739), 0.745 (95%CI 0.649-0.84), 0.795 (95%CI 0.709-0.88), and 0.818 (95%CI 0.739-0.897), respectively. Furthermore, the combination of these 4 miRNAs exhibited better diagnostic performance compared with any individual miRNA, with an AUC of 0.837 (95%CI 0.763-0.911). These data indicate that plasma let-7i-5p, miR-32-3p, miR-3149, and miR-26a-5p have promising diagnostic value for severe CAD.

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References

Zhong X, Zhang L, Li Y, Li P, Li J, Cheng G . Kaempferol alleviates ox-LDL-induced apoptosis by up-regulation of miR-26a-5p via inhibiting TLR4/NF-κB pathway in human endothelial cells. Biomed Pharmacother. 2018; 108:1783-1789. DOI: 10.1016/j.biopha.2018.09.175. View

Zuo K, Zhi K, Zhang X, Lu C, Wang S, Li M . A dysregulated microRNA-26a/EphA2 axis impairs endothelial progenitor cell function via the p38 MAPK/VEGF pathway. Cell Physiol Biochem. 2015; 35(2):477-88. DOI: 10.1159/000369713. View

Kong B, Qin Z, Ye Z, Yang X, Li L, Su Q . microRNA-26a-5p affects myocardial injury induced by coronary microembolization by modulating HMGA1. J Cell Biochem. 2019; 120(6):10756-10766. DOI: 10.1002/jcb.28367. View

Peng J, He X, Zhang L, Liu P . MicroRNA‑26a protects vascular smooth muscle cells against H2O2‑induced injury through activation of the PTEN/AKT/mTOR pathway. Int J Mol Med. 2018; 42(3):1367-1378. PMC: 6089772. DOI: 10.3892/ijmm.2018.3746. View

Nguyen T, Su C, Singh M . inhibition enhances progesterone-induced functional recovery in a mouse model of ischemia. Proc Natl Acad Sci U S A. 2018; 115(41):E9668-E9677. PMC: 6187141. DOI: 10.1073/pnas.1803384115. View

Kanuri S, Ipe J, Kassab K, Gao H, Liu Y, Skaar T . Next generation MicroRNA sequencing to identify coronary artery disease patients at risk of recurrent myocardial infarction. Atherosclerosis. 2018; 278:232-239. PMC: 6350783. DOI: 10.1016/j.atherosclerosis.2018.09.021. View

Li P, Li S, Liu M, Ruan J, Wang Z, Xie W . Value of the expression of miR-208, miR-494, miR-499 and miR-1303 in early diagnosis of acute myocardial infarction. Life Sci. 2019; 232:116547. DOI: 10.1016/j.lfs.2019.116547. View

Zhou M, Wang H, Zhu J, Chen W, Wang L, Liu S . Cause-specific mortality for 240 causes in China during 1990-2013: a systematic subnational analysis for the Global Burden of Disease Study 2013. Lancet. 2015; 387(10015):251-72. DOI: 10.1016/S0140-6736(15)00551-6. View

Satoh M, Minami Y, Takahashi Y, Tabuchi T, Nakamura M . A cellular microRNA, let-7i, is a novel biomarker for clinical outcome in patients with dilated cardiomyopathy. J Card Fail. 2011; 17(11):923-9. DOI: 10.1016/j.cardfail.2011.07.012. View

10.

Yu H, Duan B, Jiang L, Lin M, Sheng H, Huang J . Serum miR-200c and clinical outcome of patients with advanced esophageal squamous cancer receiving platinum-based chemotherapy. Am J Transl Res. 2013; 6(1):71-7. PMC: 3853426. View

11.

. Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. 2018; 392(10159):1736-1788. PMC: 6227606. DOI: 10.1016/S0140-6736(18)32203-7. View

12.

Li H, Zhao X, Liu Y, Meng Z, Wang D, Yang F . Plasma MicroRNA-126-5p is Associated with the Complexity and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris. Cell Physiol Biochem. 2016; 39(3):837-46. DOI: 10.1159/000447794. View

13.

Bao M, Feng X, Zhang Y, Lou X, Cheng Y, Zhou H . Let-7 in cardiovascular diseases, heart development and cardiovascular differentiation from stem cells. Int J Mol Sci. 2013; 14(11):23086-102. PMC: 3856107. DOI: 10.3390/ijms141123086. View

14.

Icli B, Wara A, Moslehi J, Sun X, Plovie E, Cahill M . MicroRNA-26a regulates pathological and physiological angiogenesis by targeting BMP/SMAD1 signaling. Circ Res. 2013; 113(11):1231-41. PMC: 4068743. DOI: 10.1161/CIRCRESAHA.113.301780. View

15.

Shen J, Xing W, Liu R, Zhang Y, Xie C, Gong F . MiR-32-5p influences high glucose-induced cardiac fibroblast proliferation and phenotypic alteration by inhibiting DUSP1. BMC Mol Biol. 2019; 20(1):21. PMC: 6704591. DOI: 10.1186/s12867-019-0135-x. View

16.

Liu X, Duan B, Dong Y, He C, Zhou H, Sheng H . MicroRNA-139-3p indicates a poor prognosis of colon cancer. Int J Clin Exp Pathol. 2015; 7(11):8046-52. PMC: 4270559. View

17.

Welch R, Zalenski R, Frederick P, Malmgren J, Compton S, Grzybowski M . Prognostic value of a normal or nonspecific initial electrocardiogram in acute myocardial infarction. JAMA. 2001; 286(16):1977-84. DOI: 10.1001/jama.286.16.1977. View

18.

Liu J, Xiao X, Shen Y, Chen L, Xu C, Zhao H . MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification. PLoS One. 2017; 12(3):e0174138. PMC: 5358880. DOI: 10.1371/journal.pone.0174138. View

19.

Xu G, Cui Y, Jia Z, Yue Y, Yang S . The Values of Coronary Circulating miRNAs in Patients with Atrial Fibrillation. PLoS One. 2016; 11(11):e0166235. PMC: 5113910. DOI: 10.1371/journal.pone.0166235. View

20.

Veremeyko T, Kuznetsova I, Dukhinova M, Yung A, Kopeikina E, Barteneva N . Neuronal extracellular microRNAs miR-124 and miR-9 mediate cell-cell communication between neurons and microglia. J Neurosci Res. 2018; 97(2):162-184. PMC: 6587827. DOI: 10.1002/jnr.24344. View