Selection and Analytical Applications of Aptamers Binding Microbial Pathogens

Overview

Journal Trends Analyt Chem

Specialty Chemistry

Date 2020 Apr 15

PMID 32287535

Citations 39

Authors

Camille L A Hamula

Hongquan Zhang

Feng Li

Zhixin Wang

X Chris Le

Xing-Fang Li

Affiliations

Soon will be listed here.

Abstract

DNA aptamers specifically recognizing microbial cells and viruses have a range of analytical and therapeutic applications. This article describes recent advances in the development of aptamers targeting specific pathogens (e.g., live bacteria, whole viral particles, and virally-infected mammalian cells). Specific aptamers against pathogens have been used as affinity reagents to develop sandwich assays, to label and to image cells, to bind with cells for flow-cytometry analysis, and to act as probes for development of whole-cell biosensors. Future applications of aptamers to pathogens will benefit from recent advances in improved selection and new aptamers containing modified nucleotides, particularly slow off-rate modified aptamers (SOMAmers).

Citing Articles

A Truncated Multi-Thiol Aptamer-Based SARS-CoV-2 Electrochemical Biosensor: Towards Variant-Specific Point-of-Care Detection with Optimized Fabrication.

Molina Ramirez S, Samiseresht N, Martinez-Roque M, Catania F, Graef K, Rabe M Biosensors (Basel). 2025; 15(1).

PMID: 39852074 PMC: 11763500. DOI: 10.3390/bios15010024.

New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers.

Domsicova M, Korcekova J, Poturnayova A, Breier A Int J Mol Sci. 2024; 25(13).

PMID: 38999943 PMC: 11240909. DOI: 10.3390/ijms25136833.

Advances in aptamer-based biosensors for monitoring foodborne pathogens.

Bruce-Tagoe T, Bhaskar S, Kavle R, Jeevanandam J, Acquah C, Ohemeng-Boahen G J Food Sci Technol. 2024; 61(7):1252-1271.

PMID: 38910921 PMC: 11190136. DOI: 10.1007/s13197-023-05889-8.

Analysis of lineage-specific traits upon cell sorting.

Fernandez-Fernandez R, Lopez-Igual R, Casadesus J, Sanchez-Romero M Front Cell Infect Microbiol. 2023; 13:1146070.

PMID: 37065195 PMC: 10090396. DOI: 10.3389/fcimb.2023.1146070.

Application of a Novel Aptamer Beacon for Rapid Detection of IgG1 Antibody Drugs.

Yang K, Zheng W, Huang X, Chen K, Duan C Appl Biochem Biotechnol. 2023; 195(11):7075-7085.

PMID: 36976505 DOI: 10.1007/s12010-023-04471-4.

References

Bruno J, Carrillo M, Phillips T . In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes. Folia Microbiol (Praha). 2008; 53(4):295-302. DOI: 10.1007/s12223-008-0046-6. View

Jeong Y, Kim S, Jang S, You J . Examination of specific binding activity of aptamer RNAs to the HIV-NC by using a cell-based in vivo assay for protein-RNA interaction. BMB Rep. 2008; 41(7):511-5. DOI: 10.5483/bmbrep.2008.41.7.511. View

Tang Z, Parekh P, Turner P, Moyer R, Tan W . Generating aptamers for recognition of virus-infected cells. Clin Chem. 2009; 55(4):813-22. PMC: 3869972. DOI: 10.1373/clinchem.2008.113514. View

Gold L, Ayers D, Bertino J, Bock C, Bock A, Brody E . Aptamer-based multiplexed proteomic technology for biomarker discovery. PLoS One. 2010; 5(12):e15004. PMC: 3000457. DOI: 10.1371/journal.pone.0015004. View

Xu J, Kochanek K, Murphy S, Tejada-Vera B . Deaths: final data for 2007. Natl Vital Stat Rep. 2014; 58(19):1-19. View

Weiss S, Proske D, Neumann M, Groschup M, Kretzschmar H, Famulok M . RNA aptamers specifically interact with the prion protein PrP. J Virol. 1997; 71(11):8790-7. PMC: 192345. DOI: 10.1128/JVI.71.11.8790-8797.1997. View

Vaught J, Bock C, Carter J, Fitzwater T, Otis M, Schneider D . Expanding the chemistry of DNA for in vitro selection. J Am Chem Soc. 2010; 132(12):4141-51. DOI: 10.1021/ja908035g. View

Joshi R, Janagama H, Dwivedi H, Senthil Kumar T, Jaykus L, Schefers J . Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars. Mol Cell Probes. 2008; 23(1):20-8. DOI: 10.1016/j.mcp.2008.10.006. View

Lautner G, Balogh Z, Bardoczy V, Meszaros T, Gyurcsanyi R . Aptamer-based biochips for label-free detection of plant virus coat proteins by SPR imaging. Analyst. 2010; 135(5):918-26. DOI: 10.1039/b922829b. View

10.

Nitsche A, Kurth A, Dunkhorst A, Panke O, Sielaff H, Junge W . One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX. BMC Biotechnol. 2007; 7:48. PMC: 1994675. DOI: 10.1186/1472-6750-7-48. View

11.

Li N, Ebright J, Stovall G, Chen X, Nguyen H, Singh A . Technical and biological issues relevant to cell typing with aptamers. J Proteome Res. 2009; 8(5):2438-48. DOI: 10.1021/pr801048z. View

12.

Hamula C, Zhang H, Guan L, Li X, Le X . Selection of aptamers against live bacterial cells. Anal Chem. 2008; 80(20):7812-9. DOI: 10.1021/ac801272s. View

13.

Zelada-Guillen G, Riu J, Duzgun A, Rius F . Immediate detection of living bacteria at ultralow concentrations using a carbon nanotube based potentiometric aptasensor. Angew Chem Int Ed Engl. 2009; 48(40):7334-7. DOI: 10.1002/anie.200902090. View

14.

Takemura K, Wang P, Vorberg I, Surewicz W, Priola S, Kanthasamy A . DNA aptamers that bind to PrP(C) and not PrP(Sc) show sequence and structure specificity. Exp Biol Med (Maywood). 2006; 231(2):204-14. DOI: 10.1177/153537020623100211. View

15.

Gopinath S, Misono T, Kawasaki K, Mizuno T, Imai M, Odagiri T . An RNA aptamer that distinguishes between closely related human influenza viruses and inhibits haemagglutinin-mediated membrane fusion. J Gen Virol. 2006; 87(Pt 3):479-487. DOI: 10.1099/vir.0.81508-0. View

16.

Hamula C, Le X, Li X . DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Anal Chem. 2011; 83(10):3640-7. DOI: 10.1021/ac200575e. View

17.

Pan Q, Zhang X, Wu H, He P, Wang F, Zhang M . Aptamers that preferentially bind type IVB pili and inhibit human monocytic-cell invasion by Salmonella enterica serovar typhi. Antimicrob Agents Chemother. 2005; 49(10):4052-60. PMC: 1251553. DOI: 10.1128/AAC.49.10.4052-4060.2005. View

18.

Ohk S, Koo O, Sen T, Yamamoto C, Bhunia A . Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J Appl Microbiol. 2010; 109(3):808-17. DOI: 10.1111/j.1365-2672.2010.04709.x. View

19.

Rhie A, Kirby L, Sayer N, Wellesley R, Disterer P, Sylvester I . Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion. J Biol Chem. 2003; 278(41):39697-705. DOI: 10.1074/jbc.M305297200. View

20.

Bruno J, Carrillo M, Phillips T . Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection. J Biomol Tech. 2009; 19(2):109-15. PMC: 2361164. View