In Vitro Antibacterial Effects of Antilipopolysaccharide DNA Aptamer-C1qrs Complexes

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2008 Sep 2

PMID 18759112

Citations 24

Authors

J G Bruno

M P Carrillo

T Phillips

Affiliations

Soon will be listed here.

Abstract

DNA aptamers were developed against lipopolysaccharide (LPS) from E. coli O111:B4 and shown to bind both LPS and E. coli by a colorimetric enzyme-based microplate assay. The polyclonal aptamers were coupled to human C1qrs protein either directly using a bifunctional linker or indirectly using biotinylated aptamers and a streptavidin-C1qrs complex. Both systems significantly reduced colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10x dilutions of the bacteria in the presence of human serum; it was diluted 1: 10(3) in order to avoid significant bacterial lysis by the competing alternate pathway of complement activation. A number of candidate DNA aptamer sequences were cloned and sequenced from the anti-LPS aptamer library for future screening of antibacterial or "antibiotic" potential and to aid in eventual development of an alternative therapy for antibiotic-resistant bacterial infections.

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