Dependency of the Optical Scattering Properties of Human Milk on Casein Content and Common Sample Preparation Methods

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2020 Apr 13

PMID 32279467

Citations 1

Authors

Colin Veenstra

Dayna E Every

Wilma Petersen

Johannes B van Goudoever

Wiendelt Steenbergen

Nienke Bosschaart

Affiliations

Soon will be listed here.

Abstract

Significance: Quantifying human milk composition is important for daily nutritional management in neonatal intensive cares worldwide. Photonic solutions based on visible light can potentially aid in this analysis, as energy content of human milk depends largely on fat content, and the optical scattering properties of human milk predominantly depend on the size and concentration of fat globules. However, it is expected that human milk scattering changes upon homogenization, routinely done before analysis, which may affect fat globule size.

Aim: The first aim of this study was to investigate how the most common homogenization methods (gently inverting by hand, vortexing, and sonication) affect the optical properties of human milk. The second aim was to estimate the scattering contribution of casein micelles, the second most dominant scatterers in human milk.

Approach: We combined diffuse reflectance spectroscopy with spectroscopic optical coherence tomography to measure the scattering coefficient μs, reduced scattering coefficient μs', and anisotropy g between 450 and 600 nm.

Results: Sonication induced the strongest changes in μs, μs', and g compared to the gently inverted samples (203%, 202%, and 7%, respectively, at 550 nm), but also vortexing changed μs' with 20%. Although casein micelles only showed a modest contribution to μs and g at 550 nm (7% and 1%, respectively), their contribution to μs' was 29%.

Conclusions: The scattering properties of human milk strongly depend on the homogenization method that is employed, and gentle inversion should be the preferred method. The contribution of casein micelles was relatively small for μs and g but considerably larger for μs'.

Citing Articles

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References

Aernouts B, Polshin E, Lammertyn J, Saeys W . Visible and near-infrared spectroscopic analysis of raw milk for cow health monitoring: reflectance or transmittance?. J Dairy Sci. 2011; 94(11):5315-29. DOI: 10.3168/jds.2011-4354. View

Jaaskelainen A, Peiponen K, Raty J . On reflectometric measurement of a refractive index of milk. J Dairy Sci. 2001; 84(1):38-43. DOI: 10.3168/jds.S0022-0302(01)74449-9. View

Cheong F, Xiao K, Grier D . Technical note: characterizing individual milk fat globules with holographic video microscopy. J Dairy Sci. 2008; 92(1):95-9. DOI: 10.3168/jds.2008-1361. View

Bosschaart N, Mentink R, Kok J, van Leeuwen T, Aalders M . Optical properties of neonatal skin measured in vivo as a function of age and skin pigmentation. J Biomed Opt. 2011; 16(9):097003. DOI: 10.1117/1.3622629. View

Veenstra C, Lenferink A, Petersen W, Steenbergen W, Bosschaart N . Optical properties of human milk. Biomed Opt Express. 2019; 10(8):4059-4074. PMC: 6701531. DOI: 10.1364/BOE.10.004059. View

Udabage P, McKinnon I, Augustin M . Mineral and casein equilibria in milk: effects of added salts and calcium-chelating agents. J Dairy Res. 2000; 67(3):361-70. DOI: 10.1017/s0022029900004271. View

Victora C, Bahl R, Barros A, Franca G, Horton S, Krasevec J . Breastfeeding in the 21st century: epidemiology, mechanisms, and lifelong effect. Lancet. 2016; 387(10017):475-90. DOI: 10.1016/S0140-6736(15)01024-7. View

Aernouts B, Van Beers R, Watte R, Huybrechts T, Jordens J, Vermeulen D . Effect of ultrasonic homogenization on the Vis/NIR bulk optical properties of milk. Colloids Surf B Biointerfaces. 2015; 126:510-9. DOI: 10.1016/j.colsurfb.2015.01.004. View

Bosschaart N, Leproux A, Abdalsalam O, Chen W, McLaren C, Tromberg B . Diffuse optical spectroscopic imaging for the investigation of human lactation physiology: a case study on mammary involution. J Biomed Opt. 2019; 24(5):1-8. PMC: 6532824. DOI: 10.1117/1.JBO.24.5.056006. View

10.

Bosschaart N, Faber D, van Leeuwen T, Aalders M . Measurements of wavelength dependent scattering and backscattering coefficients by low-coherence spectroscopy. J Biomed Opt. 2011; 16(3):030503. DOI: 10.1117/1.3553005. View

11.

Stocker S, Foschum F, Krauter P, Bergmann F, Hohmann A, Scalfi Happ C . Broadband Optical Properties of Milk. Appl Spectrosc. 2016; 71(5):951-962. DOI: 10.1177/0003702816666289. View

12.

Li R, Fein S, Chen J, Grummer-Strawn L . Why mothers stop breastfeeding: mothers' self-reported reasons for stopping during the first year. Pediatrics. 2008; 122 Suppl 2:S69-76. DOI: 10.1542/peds.2008-1315i. View

13.

Takahashi K, Mizuno K, Itabashi K . The freeze-thaw process and long intervals after fortification denature human milk fat globules. Am J Perinatol. 2011; 29(4):283-8. DOI: 10.1055/s-0031-1295659. View

14.

Rollins N, Bhandari N, Hajeebhoy N, Horton S, Lutter C, Martines J . Why invest, and what it will take to improve breastfeeding practices?. Lancet. 2016; 387(10017):491-504. DOI: 10.1016/S0140-6736(15)01044-2. View

15.

Arslanoglu S, Moro G, Ziegler E . Adjustable fortification of human milk fed to preterm infants: does it make a difference?. J Perinatol. 2006; 26(10):614-21. DOI: 10.1038/sj.jp.7211571. View

16.

Farrell T, Patterson M, Wilson B . A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivo. Med Phys. 1992; 19(4):879-88. DOI: 10.1118/1.596777. View

17.

Hinde K, German J . Food in an evolutionary context: insights from mother's milk. J Sci Food Agric. 2012; 92(11):2219-23. PMC: 3836823. DOI: 10.1002/jsfa.5720. View

18.

Fusch G, Rochow N, Choi A, Fusch S, Poeschl S, Ubah A . Rapid measurement of macronutrients in breast milk: How reliable are infrared milk analyzers?. Clin Nutr. 2014; 34(3):465-76. PMC: 5050037. DOI: 10.1016/j.clnu.2014.05.005. View

19.

Ullah R, Khan S, Javaid S, Ali H, Bilal M, Saleem M . Raman spectroscopy combined with a support vector machine for differentiating between feeding male and female infants mother's milk. Biomed Opt Express. 2018; 9(2):844-851. PMC: 5854083. DOI: 10.1364/BOE.9.000844. View

20.

Veenstra C, Petersen W, Vellekoop I, Steenbergen W, Bosschaart N . Spatially confined quantification of bilirubin concentrations by spectroscopic visible-light optical coherence tomography. Biomed Opt Express. 2018; 9(8):3581-3589. PMC: 6191639. DOI: 10.1364/BOE.9.003581. View