Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2020 Apr 12

PMID 32276503

Citations 8

Authors

Joery De Kock

Robim Marcelino Rodrigues

Steven Branson

Lieven Verhoye

Haaike Colemonts-Vroninks

Matthias Rombaut

Joost Boeckmans

Jessie Neuckermans

Sien Lequeue

Karolien Buyl

Makram Merimi

Douaa Moussa Agha

Veerle De Boe

Laurence Lagneaux

Philip Meuleman

Tamara Vanhaecke

Mehdi Najar

Affiliations

Soon will be listed here.

Abstract

Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.

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