Hydroalcoholic Extract Induces Apoptosis and Alters and Gene Expression in Hela Cervical Cancer Cells

Overview

Journal Rep Biochem Mol Biol

Specialty Biochemistry

Date 2020 Apr 11

PMID 32274404

Citations 7

Authors

Mehdi Rezai

Ramin Saravani

Saman Sargazi

Mahdiyeh Moudi

Mahdieh Jafari Shahroudi

Roya Saravani

Affiliations

Soon will be listed here.

Abstract

Background: Inappropriate activation of the proto-oncogene LIN28B and inactivation of the p53 tumor suppressor, have been shown to have a critical role in tumorigenesis. Previous research has shown therapeutic potential for the use of herbal plants as an alternative strategy for cancer treatment. C. Koch is a plant that has been traditionally used for its medicinal properties. The aim of this study was to investigate the cytotoxic and apoptosis-inducing effect of hydroalcoholic extract () on HeLa cervical cancer cells and its effect on and expression.

Methods: The cytotoxic activity of was evaluated on HeLa cells using a trypan blue exclusion assay. The Annexin V/PI double staining assay was used to evaluate the apoptosis-inducing effect of the extract. The expression of and mRNA was measured using the real-time-PCR method.

Results: Treatment with was shown to induce cytotoxicity in both time and concentration-dependent manners (P<0.05). The proposition of HeLa cells undergoing apoptosis increased with increasing concentrations of (P<0.05). The mRNA levels of p53 increased following 12, 24, and 48 hours of AWHE treatment whereas the mRNA levels of were significantly decreased after 4 to 12 hours of treatment (p<0.05).

Conclusion: Our findings confirmed the pro-apoptotic function of on the cervical cancer HeLa cell line. This indicates that targeting the LIN28B signaling cascade may be a promising therapeutic strategy for cervical cancer. Further research is required to understand the therapeutic effects of in primary human cervical cancer cells and a pre-clinical cervical cancer model.

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