First Report of VIM Metallo-β-lactamase Production in and Clinical Isolates from Gaza Strip, Palestine

Overview

Journal Germs

Specialty Infectious Diseases

Date 2020 Apr 11

PMID 32274356

Citations 1

Authors

Ghassan Tayh

Daram Nagarjuna

Rym Ben Sallem

Vivek Verma

Sarra Chairat

Abdellatif Boudabous

Manisha Yadav

Karim Ben Slama

Affiliations

Soon will be listed here.

Abstract

Introduction: Even though the increasing incidence of VIM-producing and has been reported worldwide, studies are still lacking in Palestine. The aim of this study was to screen carbapenem-resistant and bacteria in the Gaza Strip, Palestine and further to characterize carbapenemase-producing isolates.

Methods: A total of 69 and 27 isolates were obtained from three Gaza hospitals and recovered from urine, wound swabs, blood and ear discharge. The screening for metallo-β-lactamases (MBLs) was performed by using the imipenem-EDTA disc synergy test. The detection of β-lactamases genes, detection of non-β-lactam genes and the characterization of integrons were performed by PCR and sequencing. The clonal relationship among the isolates was determined by pulsed-field gel electrophoresis (PFGE).

Results: Our study showed that 4 (5.8%) and 5 (18.5%) were positive by the imipenem-EDTA disc synergy test. was detected in six isolates and was identified in three isolates. The β-lactamases genes in the VIM-producing isolates were (n=3), (n=1), (n=3), (n=1), (n=1) and (n=1). -Ib-cr gene was confirmed in four and in two isolates. was identified in two isolates. The class 1 integron was identified with the different gene cassette; and were identified.

Conclusions: Our study indicated for the first time the emergence of multidrug-resistant VIM-containing and isolates of clinical origin in Gaza Strip hospitals.

Citing Articles

First report of carbapenems encoding multidrug-resistant gram-negative bacteria from a pediatric hospital in Gaza Strip, Palestine.

El Aila N, Al Laham N, Doijad S, Imirzalioglu C, Mraheil M BMC Microbiol. 2024; 24(1):393.

PMID: 39379824 PMC: 11462914. DOI: 10.1186/s12866-024-03550-8.

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