Tick and Host Derived Compounds Detected in the Cement Complex Substance

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2020 Apr 9

PMID 32260542

Citations 19

Authors

Margarita Villar

Ivan Pacheco

Octavio Merino

Marinela Contreras

Lourdes Mateos-Hernandez

Eduardo Prado

Dina Karen Barros-Picanco

Jose Francisco Lima-Barbero

Sara Artigas-Jeronimo

Pilar Alberdi

Isabel G Fernandez de Mera

Agustin Estrada-Pena

Alejandro Cabezas-Cruz

Jose de la Fuente

Affiliations

Soon will be listed here.

Abstract

Ticks are obligate hematophagous arthropods and vectors of pathogens affecting human and animal health worldwide. Cement is a complex protein polymerization substance secreted by ticks with antimicrobial properties and a possible role in host attachment, sealing the feeding lesion, facilitating feeding and pathogen transmission, and protection from host immune and inflammatory responses. The biochemical properties of tick cement during feeding have not been fully characterized. In this study, we characterized the proteome of salivary glands (sialome) and cement (cementome) together with their physicochemical properties at different adult female parasitic stages. The results showed the combination of tick and host derived proteins and other biomolecules such as α-Gal in cement composition, which varied during the feeding process. We propose that these compounds may synergize in cement formation, solidification and maintenance to facilitate attachment, feeding, interference with host immune response and detachment. These results advanced our knowledge of the complex tick cement composition and suggested that tick and host derived compounds modulate cement properties throughout tick feeding.

Citing Articles

Transcriptomic landscapes reveal development-related physiological processes in the two-spotted spider mite, Tetranychus urticae.

Gao S, Niu Y, Chen L, Chen M, Bing X, Hong X Exp Appl Acarol. 2024; 93(4):743-759.

PMID: 39150623 DOI: 10.1007/s10493-024-00956-x.

Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India.

Perumalsamy N, Sharma R, Subramanian M, Nagarajan S Pathogens. 2024; 13(7).

PMID: 39057783 PMC: 11279560. DOI: 10.3390/pathogens13070556.

Allergic reactions to tick saliva components in zebrafish model.

Contreras M, Vaz-Rodrigues R, Mazuecos L, Villar M, Artigas-Jeronimo S, Gonzalez-Garcia A Parasit Vectors. 2023; 16(1):242.

PMID: 37468955 PMC: 10357745. DOI: 10.1186/s13071-023-05874-2.

Novel symbionts and potential human pathogens excavated from argasid tick microbiomes that are shaped by dual or single symbiosis.

Moustafa M, Mohamed W, Lau A, Chatanga E, Qiu Y, Hayashi N Comput Struct Biotechnol J. 2022; 20:1979-1992.

PMID: 35521555 PMC: 9062450. DOI: 10.1016/j.csbj.2022.04.020.

Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions.

Ali A, Zeb I, Alouffi A, Zahid H, Almutairi M, Alshammari F Front Cell Infect Microbiol. 2022; 12:809052.

PMID: 35372098 PMC: 8966233. DOI: 10.3389/fcimb.2022.809052.

References

Molaei G, Little E, Williams S, Stafford K . Bracing for the Worst - Range Expansion of the Lone Star Tick in the Northeastern United States. N Engl J Med. 2019; 381(23):2189-2192. DOI: 10.1056/NEJMp1911661. View

Diaz-Martin V, Manzano-Roman R, Valero L, Oleaga A, Encinas-Grandes A, Perez-Sanchez R . An insight into the proteome of the saliva of the argasid tick Ornithodoros moubata reveals important differences in saliva protein composition between the sexes. J Proteomics. 2013; 80:216-35. DOI: 10.1016/j.jprot.2013.01.015. View

Narasimhan S, Booth C, DePonte K, Wu M, Liang X, Mohanty S . Host-specific expression of Ixodes scapularis salivary genes. Ticks Tick Borne Dis. 2018; 10(2):386-397. DOI: 10.1016/j.ttbdis.2018.12.001. View

Hajdusek O, Sima R, Ayllon N, Jalovecka M, Perner J, de la Fuente J . Interaction of the tick immune system with transmitted pathogens. Front Cell Infect Microbiol. 2013; 3:26. PMC: 3712896. DOI: 10.3389/fcimb.2013.00026. View

Alberdi P, Cabezas-Cruz A, Prados P, Rayo M, Artigas-Jeronimo S, de la Fuente J . The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells. Sci Rep. 2019; 9(1):13236. PMC: 6744499. DOI: 10.1038/s41598-019-49766-x. View

de la Fuente J . Controlling ticks and tick-borne diseases…looking forward. Ticks Tick Borne Dis. 2018; 9(5):1354-1357. DOI: 10.1016/j.ttbdis.2018.04.001. View

Bullard R, Allen P, Chao C, Douglas J, Das P, Morgan S . Structural characterization of tick cement cones collected from in vivo and artificial membrane blood-fed Lone Star ticks (Amblyomma americanum). Ticks Tick Borne Dis. 2016; 7(5):880-892. PMC: 5460760. DOI: 10.1016/j.ttbdis.2016.04.006. View

Kim T, Tirloni L, Pinto A, Diedrich J, Moresco J, Yates 3rd J . Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding. PLoS Negl Trop Dis. 2020; 14(2):e0007758. PMC: 7041860. DOI: 10.1371/journal.pntd.0007758. View

Kim T, Curran J, Mulenga A . Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability. J Exp Biol. 2014; 217(Pt 19):3493-503. PMC: 4182284. DOI: 10.1242/jeb.107979. View

10.

Stewart R, Ransom T, Hlady V . Natural Underwater Adhesives. J Polym Sci B Polym Phys. 2011; 49(11):757-771. PMC: 3104275. DOI: 10.1002/polb.22256. View

11.

Hollmann T, Kim T, Tirloni L, Radulovic Z, Pinto A, Diedrich J . Identification and characterization of proteins in the Amblyomma americanum tick cement cone. Int J Parasitol. 2017; 48(3-4):211-224. PMC: 5844823. DOI: 10.1016/j.ijpara.2017.08.018. View

12.

Merino O, Antunes S, Mosqueda J, Moreno-Cid J, de la Lastra J, Rosario-Cruz R . Vaccination with proteins involved in tick-pathogen interactions reduces vector infestations and pathogen infection. Vaccine. 2013; 31(49):5889-96. DOI: 10.1016/j.vaccine.2013.09.037. View

13.

Commins S, Satinover S, Hosen J, Mozena J, Borish L, Lewis B . Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-alpha-1,3-galactose. J Allergy Clin Immunol. 2008; 123(2):426-33. PMC: 3324851. DOI: 10.1016/j.jaci.2008.10.052. View

14.

Moreno-Cid J, de la Lastra J, Villar M, Jimenez M, Pinal R, Estrada-Pena A . Control of multiple arthropod vector infestations with subolesin/akirin vaccines. Vaccine. 2013; 31(8):1187-96. DOI: 10.1016/j.vaccine.2012.12.073. View

15.

de la Fuente J, Estrada-Pena A . Why New Vaccines for the Control of Ectoparasite Vectors Have Not Been Registered and Commercialized?. Vaccines (Basel). 2019; 7(3). PMC: 6789832. DOI: 10.3390/vaccines7030075. View

16.

Almazan C, Moreno-Cantu O, Moreno-Cid J, Galindo R, Canales M, Villar M . Control of tick infestations in cattle vaccinated with bacterial membranes containing surface-exposed tick protective antigens. Vaccine. 2011; 30(2):265-72. DOI: 10.1016/j.vaccine.2011.10.102. View

17.

Kim T, Tirloni L, Pinto A, Moresco J, Yates 3rd J, da Silva Vaz Jr I . Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding. PLoS Negl Trop Dis. 2016; 10(1):e0004323. PMC: 4709002. DOI: 10.1371/journal.pntd.0004323. View

18.

Layeghifard M, Hwang D, Guttman D . Disentangling Interactions in the Microbiome: A Network Perspective. Trends Microbiol. 2016; 25(3):217-228. PMC: 7172547. DOI: 10.1016/j.tim.2016.11.008. View

19.

Perner J, Kropackova S, Kopacek P, Ribeiro J . Sialome diversity of ticks revealed by RNAseq of single tick salivary glands. PLoS Negl Trop Dis. 2018; 12(4):e0006410. PMC: 5919021. DOI: 10.1371/journal.pntd.0006410. View

20.

Huang H, Chen W, Wu J . Total protein extraction for metaproteomics analysis of methane producing biofilm: the effects of detergents. Int J Mol Sci. 2014; 15(6):10169-84. PMC: 4100146. DOI: 10.3390/ijms150610169. View