Comparative Proteomic Analysis of Sensu Lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Infection

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2021 Feb 15

PMID 33585280

Citations 1

Authors

Gustavo Seron Sanches

Margarita Villar

Joana Couto

Joana Ferrolho

Isabel G Fernandez de Mera

Marcos Rogerio Andre

Darci Moraes Barros-Battesti

Rosangela Zacarias Machado

Gervasio Henrique Bechara

Lourdes Mateos-Hernandez

Jose de la Fuente

Sandra Antunes

Ana Domingos

Affiliations

Soon will be listed here.

Abstract

The tick vector is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the "temperate" and "tropical" lineages of (s.l.) is the vector competence to , suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the tropical and temperate lineages and compares their proteomic profile during infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control.

Citing Articles

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