» Articles » PMID: 32248725

Zinc Regulates Reactive Oxygen Species Generation in Platelets

Overview
Journal Platelets
Publisher Informa Healthcare
Specialty Hematology
Date 2020 Apr 7
PMID 32248725
Citations 14
Authors
Affiliations
Soon will be listed here.
Abstract

Vascular complications resulting from atherosclerosis development are a major cause of death. Reactive oxygen species (ROS) are produced by platelets during activation, and have been demonstrated to positively regulate platelet activatory responses. Zn is also an important hemostatic cofactor in platelets, acting both as a platelet agonist and second messenger. Whilst the effect of Zn-dependent signaling mechanisms on ROS production in nucleated cells has been demonstrated, comparable roles in platelets have yet to be investigated. In this study we investigated the relationship between fluctuations in cytosolic Zn [Zn] and platelet ROS production. Agonist-evoked ROS production, GSH levels and GPx activity are abrogated in platelets treated with the Zn-chelator, TPEN. Conversely, increasing platelet [Zn] using Zn ionophores potentiated ROS generation and decreased GSH levels and GPx activity. Zn-dependent ROS production was sensitive to pretreatment with DPI or mitoTEMPO, NADPH oxidase and mitochondria inhibitors respectively. Increasing [Zn] resulted in increases of Erk1/2 and JNK phosphorylation. Our data are consistent with a functional association between [Zn] and ROS production in platelets that could influence thrombus formation in a clinical context.

Citing Articles

SARS-CoV-2 drug resistance and therapeutic approaches.

Batool S, Chokkakula S, Jeong J, Baek Y, Song M Heliyon. 2025; 11(2):e41980.

PMID: 39897928 PMC: 11786845. DOI: 10.1016/j.heliyon.2025.e41980.


Clays and Wound Healing.

Tian G, Wang Z, Huang Z, Xie Z, Xia L, Zhang Y Materials (Basel). 2024; 17(7).

PMID: 38612205 PMC: 11012786. DOI: 10.3390/ma17071691.


Utilization of a zinc reporter reveals the complexities of fungal sensing of metal deprivation.

Blancett L, Evans H, Candor K, Buesing W, Figueroa J, Deepe Jr G mSphere. 2024; 9(2):e0070423.

PMID: 38259064 PMC: 10900905. DOI: 10.1128/msphere.00704-23.


Delineating Zinc Influx Mechanisms during Platelet Activation.

Kuravi S, Ahmed N, Taylor K, Capes E, Bye A, Unsworth A Int J Mol Sci. 2023; 24(14).

PMID: 37511448 PMC: 10380784. DOI: 10.3390/ijms241411689.


Loss of zinc transporters ZIP1 and ZIP3 augments platelet reactivity in response to thrombin and accelerates thrombus formation .

Elgheznawy A, Oftering P, Englert M, Mott K, Kaiser F, Kusch C Front Immunol. 2023; 14:1197894.

PMID: 37359521 PMC: 10285393. DOI: 10.3389/fimmu.2023.1197894.