Chronic Regulation of MiR-124-3p in the Perilesional Cortex After Experimental and Human TBI
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Chemistry
Molecular Biology
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Traumatic brain injury (TBI) dysregulates microRNAs, which are the master regulators of gene expression. Here we investigated the changes in a brain-enriched miR-124-3p, which is known to associate with major post-injury pathologies, such as neuroinflammation. RT-qPCR of the rat tissue sampled at 7 d and 3 months in the perilesional cortex adjacent to the necrotic lesion core (aPeCx) revealed downregulation of miR-124-3p at 7 d (fold-change (FC) 0.13, < 0.05 compared with control) and 3 months (FC 0.40, < 0.05) post-TBI. In situ hybridization confirmed the downregulation of miR-124-3p at 7 d and 3 months post-TBI in the aPeCx (both < 0.01). RT-qPCR confirmed the upregulation of the miR-124-3p target in the aPeCx at 7 d post-TBI (7-fold, < 0.05). mRNA-Seq revealed 312 downregulated and 311 upregulated miR-124 targets ( < 0.05). To investigate whether experimental findings translated to humans, we performed in situ hybridization of miR-124-3p in temporal lobe autopsy samples of TBI patients. Our data revealed downregulation of miR-124-3p in individual neurons of cortical layer III. These findings indicate a persistent downregulation of miR-124-3p in the perilesional cortex that might contribute to post-injury neurodegeneration and inflammation.
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