Comparative Genomic Analysis and Mosquito Larvicidal Activity of Four Bacillus Thuringiensis Serovar Israelensis Strains

Overview

Journal Sci Rep

Specialty Science

Date 2020 Mar 29

PMID 32218451

Citations 3

Authors

Giselly B Alves

Fernando L Melo

Eugenio E Oliveira

Khalid Haddi

Lara T M Costa

Marcelo L Dias

Fabricio S Campos

Eliseu J G Pereira

Roberto F T Correa

Sergio D Ascencio

Gil R Santos

Guy Smagghe

Bergmann M Ribeiro

Raimundo W S Aguiar

Affiliations

Soon will be listed here.

Abstract

Bacillus thuringiensis serovar israelensis (Bti) is used to control insect vectors of human and animal diseases. In the present study, the toxicity of four strains of Bti, named T0124, T0131, T0137, and T0139, toward Aedes aegypti and Culex quinquefasciatus larvae was analyzed. The T0131 strain showed the highest larvicidal activity against A. aegypti (LC = 0.015 µg/ml) and C. quinquefasciatus larvae (LC = 0.035 µg/ml) when compared to the other strains. Furthermore, the genomic sequences of the four strains were obtained and compared. These Bti strains had chromosomes sizes of approximately 5.4 Mb with GC contents of ~35% and 5472-5477 putative coding regions. Three small plasmids (5.4, 6.8, and 7.6 kb) and three large plasmids (127, 235, and 359 kb) were found in the extrachromosomal content of all four strains. The SNP-based phylogeny revealed close relationship among isolates from this study and other Bti isolates, and SNPs analysis of the plasmids 127 kb did not reveal any mutations in δ-endotoxins genes. This newly acquired sequence data for these Bti strains may be useful in the search for novel insecticidal toxins to improve existing ones or develop new strategies for the biological control of important insect vectors of human and animal diseases.

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References

Jiang D, Liu G, Gong W, Li R, Hu Y, Tu C . Complete genome sequences of classical Swine Fever virus isolates belonging to a new subgenotype, 2.1c, from hunan province, china. Genome Announc. 2013; 1(1). PMC: 3569334. DOI: 10.1128/genomeA.00080-12. View

Palma L, Munoz D, Berry C, Murillo J, Caballero P . Draft genome sequences of two Bacillus thuringiensis strains and characterization of a putative 41.9-kDa insecticidal toxin. Toxins (Basel). 2014; 6(5):1490-504. PMC: 4052248. DOI: 10.3390/toxins6051490. View

Rusconi B, Chen Y, Koenig S, El-Helow E, Eppinger M . Genome Sequence of Bacillus thuringiensis Strain Btm27, an Egyptian Isolate Highly Toxic to Cotton Leafworm. Genome Announc. 2015; 3(3). PMC: 4432336. DOI: 10.1128/genomeA.00446-15. View

Iatsenko I, Corton C, Pickard D, Dougan G, Sommer R . Draft Genome Sequence of Highly Nematicidal Bacillus thuringiensis DB27. Genome Announc. 2014; 2(1). PMC: 3931364. DOI: 10.1128/genomeA.00101-14. View

Wang P, Zhang C, Guo M, Guo S, Zhu Y, Zheng J . Complete genome sequence of Bacillus thuringiensis YBT-1518, a typical strain with high toxicity to nematodes. J Biotechnol. 2013; 171:1-2. DOI: 10.1016/j.jbiotec.2013.11.023. View

Liu X, Zhou R, Fu G, Zhang W, Min Y, Tian Y . Draft Genome Sequence of Bacillus thuringiensis NBIN-866 with High Nematocidal Activity. Genome Announc. 2014; 2(3). PMC: 4031334. DOI: 10.1128/genomeA.00429-14. View

Jeong H, Jo S, Hong C, Park J . Genome Sequence of the Endophytic Bacterium Bacillus thuringiensis Strain KB1, a Potential Biocontrol Agent against Phytopathogens. Genome Announc. 2016; 4(2). PMC: 4841131. DOI: 10.1128/genomeA.00279-16. View

Loeza-Lara P, Benintende G, Cozzi J, Ochoa-Zarzosa A, Baizabal-Aguirre V, Valdez-Alarcon J . The plasmid pBMBt1 from Bacillus thuringiensis subsp. darmstadiensis (INTA Mo14-4) replicates by the rolling-circle mechanism and encodes a novel insecticidal crystal protein-like gene. Plasmid. 2005; 54(3):229-40. DOI: 10.1016/j.plasmid.2005.04.003. View

10.

Sheppard A, Poehlein A, Rosenstiel P, Liesegang H, Schulenburg H . Complete Genome Sequence of Bacillus thuringiensis Strain 407 Cry-. Genome Announc. 2013; 1(1). PMC: 3569317. DOI: 10.1128/genomeA.00158-12. View

11.

Zorzetti J, Ricietto A, da Silva C, Wolf I, Vilas-Boas G, Neves P . Genome Sequence of the Mosquitocidal Bacillus thuringiensis Strain BR58, a Biopesticide Product Effective against the Coffee Berry Borer (Hypothenemus hampei). Genome Announc. 2015; 3(6). PMC: 4675934. DOI: 10.1128/genomeA.01232-15. View

12.

Doggett N, Stubben C, Chertkov O, Bruce D, Detter J, Johnson S . Complete Genome Sequence of Bacillus thuringiensis Serovar Israelensis Strain HD-789. Genome Announc. 2013; 1(6). PMC: 3853066. DOI: 10.1128/genomeA.01023-13. View

13.

Jeong H, Park S, Choi S . Genome Sequence of the Acrystalliferous Bacillus thuringiensis Serovar Israelensis Strain 4Q7, Widely Used as a Recombination Host. Genome Announc. 2014; 2(2). PMC: 3974936. DOI: 10.1128/genomeA.00231-14. View

14.

Alcaraz L, Moreno-Hagelsieb G, Eguiarte L, Souza V, Herrera-Estrella L, Olmedo G . Understanding the evolutionary relationships and major traits of Bacillus through comparative genomics. BMC Genomics. 2010; 11:332. PMC: 2890564. DOI: 10.1186/1471-2164-11-332. View

15.

Araujo A, Diniz D, Helvecio E, de Barros R, Oliveira C, Ayres C . The susceptibility of Aedes aegypti populations displaying temephos resistance to Bacillus thuringiensis israelensis: a basis for management. Parasit Vectors. 2014; 6(1):297. PMC: 3852962. DOI: 10.1186/1756-3305-6-297. View

16.

Frost L, Leplae R, Summers A, Toussaint A . Mobile genetic elements: the agents of open source evolution. Nat Rev Microbiol. 2005; 3(9):722-32. DOI: 10.1038/nrmicro1235. View

17.

Rudolph R . [Electron microscopy demonstration of sulphurated acid mucopolysaccharides in canine mast cell tumors, using barium chloride and ruthenium red, together with comments on the classification and differentiation of tumor cells]. Arch Exp Veterinarmed. 1971; 25(6):925-35. View

18.

Alikhan N, Petty N, Ben Zakour N, Beatson S . BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics. 2011; 12:402. PMC: 3163573. DOI: 10.1186/1471-2164-12-402. View

19.

Kaas R, Leekitcharoenphon P, Aarestrup F, Lund O . Solving the problem of comparing whole bacterial genomes across different sequencing platforms. PLoS One. 2014; 9(8):e104984. PMC: 4128722. DOI: 10.1371/journal.pone.0104984. View

20.

Jia N, Ding M, Gao F, Yuan Y . Comparative genomics analysis of the companion mechanisms of Bacillus thuringiensis Bc601 and Bacillus endophyticus Hbe603 in bacterial consortium. Sci Rep. 2016; 6:28794. PMC: 4926094. DOI: 10.1038/srep28794. View

21.

Johnson S, Daligault H, Davenport K, Jaissle J, Frey K, Ladner J . Complete genome sequences for 35 biothreat assay-relevant bacillus species. Genome Announc. 2015; 3(2). PMC: 4417687. DOI: 10.1128/genomeA.00151-15. View