Genome Sequence of Bacillus Thuringiensis Strain Btm27, an Egyptian Isolate Highly Toxic to Cotton Leafworm

Overview

Journal Genome Announc

Publisher American Society for Microbiology

Date 2015 May 16

PMID 25977430

Citations 1

Authors

Brigida Rusconi

Yue Chen

Sara S K Koenig

Ehab R El-Helow

Mark Eppinger

Affiliations

Soon will be listed here.

Abstract

Bacillus thuringiensis is a potent microbial control agent against insect pests. Here, we present the draft genome of the Egyptian strain Btm27 that shows high toxicity toward the cotton leafworm. The genome contains three insecticidal genes cry1Ac9, cry2Ab1, and vip3V that have been implicated in conferring toxicity toward lepidoptera.

Citing Articles

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains.

Alves G, Melo F, Oliveira E, Haddi K, Costa L, Dias M Sci Rep. 2020; 10(1):5518.

PMID: 32218451 PMC: 7099026. DOI: 10.1038/s41598-020-60670-7.

References

Bravo A, Gill S, Soberon M . Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007; 49(4):423-35. PMC: 1857359. DOI: 10.1016/j.toxicon.2006.11.022. View

Ye W, Zhu L, Liu Y, Crickmore N, Peng D, Ruan L . Mining new crystal protein genes from Bacillus thuringiensis on the basis of mixed plasmid-enriched genome sequencing and a computational pipeline. Appl Environ Microbiol. 2012; 78(14):4795-801. PMC: 3416374. DOI: 10.1128/AEM.00340-12. View

Goecks J, Nekrutenko A, Taylor J . Galaxy: a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences. Genome Biol. 2010; 11(8):R86. PMC: 2945788. DOI: 10.1186/gb-2010-11-8-r86. View

Seemann T . Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014; 30(14):2068-9. DOI: 10.1093/bioinformatics/btu153. View

Ibrahim M, Griko N, Junker M, Bulla L . Bacillus thuringiensis: a genomics and proteomics perspective. Bioeng Bugs. 2011; 1(1):31-50. PMC: 3035146. DOI: 10.4161/bbug.1.1.10519. View

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

Alfazairy A, El-Ahwany A, Mohamed E, Zaghloul H, El-Helow E . Microbial control of the cotton leafworm Spodoptera littoralis (Boisd.) by Egyptian Bacillus thuringiensis isolates. Folia Microbiol (Praha). 2012; 58(2):155-62. DOI: 10.1007/s12223-012-0193-7. View

Melo A, Soccol V, Soccol C . Bacillus thuringiensis: mechanism of action, resistance, and new applications: a review. Crit Rev Biotechnol. 2014; 36(2):317-26. DOI: 10.3109/07388551.2014.960793. View

de Maagd R, Bravo A, Berry C, Crickmore N, Schnepf H . Structure, diversity, and evolution of protein toxins from spore-forming entomopathogenic bacteria. Annu Rev Genet. 2003; 37:409-33. DOI: 10.1146/annurev.genet.37.110801.143042. View

10.

Sanahuja G, Banakar R, Twyman R, Capell T, Christou P . Bacillus thuringiensis: a century of research, development and commercial applications. Plant Biotechnol J. 2011; 9(3):283-300. DOI: 10.1111/j.1467-7652.2011.00595.x. View

11.

Crickmore N, Zeigler D, Feitelson J, Schnepf E, Van Rie J, Lereclus D . Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev. 1998; 62(3):807-13. PMC: 98935. DOI: 10.1128/MMBR.62.3.807-813.1998. View

12.

Vu K, Yan S, Tyagi R, Valero J, Surampalli R . Induced production of chitinase to enhance entomotoxicity of Bacillus thuringiensis employing starch industry wastewater as a substrate. Bioresour Technol. 2009; 100(21):5260-9. DOI: 10.1016/j.biortech.2009.03.084. View

13.

Doss V, Kumar K, Jayakumar R, Sekar V . Cloning and expression of the vegetative insecticidal protein (vip3V) gene of Bacillus thuringiensis in Escherichia coli. Protein Expr Purif. 2002; 26(1):82-8. DOI: 10.1016/s1046-5928(02)00515-6. View

14.

Bankevich A, Nurk S, Antipov D, Gurevich A, Dvorkin M, Kulikov A . SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012; 19(5):455-77. PMC: 3342519. DOI: 10.1089/cmb.2012.0021. View

15.

. Activities at the Universal Protein Resource (UniProt). Nucleic Acids Res. 2013; 42(Database issue):D191-8. PMC: 3965022. DOI: 10.1093/nar/gkt1140. View