Characterization of Flavonoid Compounds in Common Swedish Berry Species

Overview

Journal Foods

Specialty Biotechnology

Date 2020 Mar 25

PMID 32204535

Citations 12

Authors

Jiyun Liu

Mohammed E Hefni

Cornelia M Witthoft

Affiliations

Soon will be listed here.

Abstract

Berries are considered an ideal source of polyphenols, especially from the flavonoid group. In this study, we examined the flavonoid content in 16 varieties of Swedish lingonberry, raspberry, blueberry, and strawberry. Nineteen flavonoids were simultaneously quantified using external standards. An additional 29 flavonoids were tentatively identified using MS as no standards were available. Quantification was done using HPLC-UV after optimization of chromatographic and extraction procedures. The method showed high linearity within the range of 2-100 μg/mL (correlation co-efficient >0.999), intra- and inter-day precision of 1.7-7.3% and average recovery above 84% for all compounds. Blueberries and lingonberries were found to contain higher contents of flavonoids (1100 mg/100 g dry weight) than raspberries and strawberries (500 mg/100 g dry weight). Anthocyanins were the dominant flavonoids in all berries. The tentatively characterized compounds contribute 18%, 29%, 61%, and 67% of the total flavonoid content in strawberries, lingonberries, raspberries, and blueberries, respectively. Overall, Swedish berries were shown to be good sources of polyphenols.

Citing Articles

Metabolomics insights into the protective molecular mechanism of Vaccinium myrtillus against oxidative stress in intestinal cells.

Novi S, Caponigro V, Miranda M, Aquino G, Carri M, Salviati E Sci Rep. 2025; 15(1):8643.

PMID: 40082563 PMC: 11906781. DOI: 10.1038/s41598-025-93722-x.

Lingonberry (- L.) Skin Extract Prevents Weight Gain and Hyperglycemia in High-Fat Diet-Induced Model of Obesity in Mice.

Ryyti R, Hamalainen M, Tolonen T, Maki M, Jaakkola M, Peltola R Nutrients. 2024; 16(13).

PMID: 38999854 PMC: 11243352. DOI: 10.3390/nu16132107.

Phenolic Compounds Known to Be Present in Lingonberry ( L.) Enhance Macrophage Polarization towards the Anti-Inflammatory M2 Phenotype.

Ryyti R, Hamalainen M, Leppanen T, Peltola R, Moilanen E Biomedicines. 2022; 10(12).

PMID: 36551801 PMC: 9776286. DOI: 10.3390/biomedicines10123045.

Metabolomic Profiling, Antioxidant and Enzyme Inhibition Properties and Molecular Docking Analysis of Antarctic Lichens.

Torres-Benitez A, Ortega-Valencia J, Sanchez M, Divakar P, Simirgiotis M, Gomez-Serranillos M Molecules. 2022; 27(22).

PMID: 36432187 PMC: 9692326. DOI: 10.3390/molecules27228086.

Dried Bilberry ( L.) Alleviates the Inflammation and Adverse Metabolic Effects Caused by a High-Fat Diet in a Mouse Model of Obesity.

Pemmari T, Hamalainen M, Ryyti R, Peltola R, Moilanen E Int J Mol Sci. 2022; 23(19).

PMID: 36232316 PMC: 9569776. DOI: 10.3390/ijms231911021.

References

Kylli P, Nohynek L, Puupponen-Pimia R, Westerlund-Wikstrom B, Leppanen T, Welling J . Lingonberry (Vaccinium vitis-idaea) and European cranberry (Vaccinium microcarpon) proanthocyanidins: isolation, identification, and bioactivities. J Agric Food Chem. 2011; 59(7):3373-84. DOI: 10.1021/jf104621e. View

Lei F, Zhang X, Wang W, Xing D, Xie W, Su H . Evidence of anti-obesity effects of the pomegranate leaf extract in high-fat diet induced obese mice. Int J Obes (Lond). 2007; 31(6):1023-9. DOI: 10.1038/sj.ijo.0803502. View

Rodrigues Sa R, da Cruz Caldas J, Santana D, Lopes M, Dos Santos W, Gracas Andrade Korn M . Multielementar/centesimal composition and determination of bioactive phenolics in dried fruits and capsules containing Goji berries (Lycium barbarum L.). Food Chem. 2018; 273:15-23. DOI: 10.1016/j.foodchem.2018.05.124. View

Wang S, Zheng W, Galletta G . Cultural system affects fruit quality and antioxidant capacity in strawberries. J Agric Food Chem. 2002; 50(22):6534-42. DOI: 10.1021/jf020614i. View

de Souza D, Willems J, Low N . Phenolic composition and antioxidant activities of saskatoon berry fruit and pomace. Food Chem. 2019; 290:168-177. DOI: 10.1016/j.foodchem.2019.03.077. View

Aaby K, Ekeberg D, Skrede G . Characterization of phenolic compounds in strawberry (Fragaria x ananassa) fruits by different HPLC detectors and contribution of individual compounds to total antioxidant capacity. J Agric Food Chem. 2007; 55(11):4395-406. DOI: 10.1021/jf0702592. View

Tian Y, Liimatainen J, Alanne A, Lindstedt A, Liu P, Sinkkonen J . Phenolic compounds extracted by acidic aqueous ethanol from berries and leaves of different berry plants. Food Chem. 2016; 220:266-281. DOI: 10.1016/j.foodchem.2016.09.145. View

Glover B, Martin C . Anthocyanins. Curr Biol. 2012; 22(5):R147-50. DOI: 10.1016/j.cub.2012.01.021. View

Kajdzanoska M, Petreska J, Stefova M . Comparison of different extraction solvent mixtures for characterization of phenolic compounds in strawberries. J Agric Food Chem. 2011; 59(10):5272-8. DOI: 10.1021/jf2007826. View

10.

Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Fernandez-Gutierrez A . Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules. 2010; 15(12):8813-26. PMC: 6259353. DOI: 10.3390/molecules15128813. View

11.

Kylli P, Nohynek L, Puupponen-Pimia R, Westerlund-Wikstrom B, McDougall G, Stewart D . Rowanberry phenolics: compositional analysis and bioactivities. J Agric Food Chem. 2010; 58(22):11985-92. DOI: 10.1021/jf102739v. View

12.

Xiao J . Dietary flavonoid aglycones and their glycosides: Which show better biological significance?. Crit Rev Food Sci Nutr. 2015; 57(9):1874-1905. DOI: 10.1080/10408398.2015.1032400. View

13.

Sparzak B, Merino-Arevalo M, Vander Heyden Y, Krauze-Baranowska M, Majdan M, Fecka I . HPLC analysis of polyphenols in the fruits of Rubus idaeus L. (Rosaceae). Nat Prod Res. 2010; 24(19):1811-22. DOI: 10.1080/14786411003754231. View

14.

Zheng W, Wang S . Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. J Agric Food Chem. 2003; 51(2):502-9. DOI: 10.1021/jf020728u. View

15.

Magalhaes S, Taveira M, Cabrita A, Fonseca A, Valentao P, Andrade P . European marketable grain legume seeds: Further insight into phenolic compounds profiles. Food Chem. 2016; 215:177-84. DOI: 10.1016/j.foodchem.2016.07.152. View

16.

Arts I, van De Putte B, Hollman P . Catechin contents of foods commonly consumed in The Netherlands. 1. Fruits, vegetables, staple foods, and processed foods. J Agric Food Chem. 2000; 48(5):1746-51. DOI: 10.1021/jf000025h. View

17.

Mullen W, McGinn J, Lean M, MacLean M, Gardner P, Duthie G . Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties. J Agric Food Chem. 2002; 50(18):5191-6. DOI: 10.1021/jf020140n. View

18.

Kalia K, Sharma K, Singh H, Singh B . Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC. J Agric Food Chem. 2008; 56(21):10129-34. DOI: 10.1021/jf802188b. View

19.

Pereira G, Arruda H, Rodrigues de Morais D, Eberlin M, Pastore G . Carbohydrates, volatile and phenolic compounds composition, and antioxidant activity of calabura (Muntingia calabura L.) fruit. Food Res Int. 2018; 108:264-273. DOI: 10.1016/j.foodres.2018.03.046. View

20.

Maatta-Riihinen K, Kamal-Eldin A, Torronen A . Identification and quantification of phenolic compounds in berries of Fragaria and Rubus species (family Rosaceae). J Agric Food Chem. 2004; 52(20):6178-87. DOI: 10.1021/jf049450r. View