Clinical Significance of MiR-19b-3p in Patients with Sepsis and Its Regulatory Role in the LPS-induced Inflammatory Response

Overview

Journal Eur J Med Res

Publisher Biomed Central

Specialty General Medicine

Date 2020 Mar 20

PMID 32188465

Citations 16

Authors

HuiMin Xu

Xiuwu Liu

Huaijun Ni

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs (miRNAs) play important roles in the development and progression of sepsis. This study investigated the clinical value of miR-19b-3p in sepsis patients, and explored its role in regulating inflammatory responses in HUVECs cells.

Methods: 103 patients with sepsis and 98 healthy individuals were recruited. qRT-PCR was used for the measurement of miR-19b-3p level. Cell viability was evaluated using CCK-8. The protein levels of TNF-α and IL-6 were measured using ELISA. Receiver operating characteristic (ROC) curve and logistic regression analysis were constructed to evaluate the diagnostic and prognostic values of miR-19b-3p in sepsis patients.

Results: MiR-19b-3p level was significantly reduced in the serum from patients with sepsis compared with healthy controls (P < 0.001). Sepsis patients in the survival group had significantly high miR-19b-3p levels compared with the non-survival group (P < 0.001). MiR-19b-3p was of a good value in predicting sepsis risk, and was an independent prognostic factor for 28-day survival in sepsis patients (OR = 3.226, 95% CI 1.076-9.670, P = 0.037). MiR-19b-3p level was negatively associated with serum levels of IL-6 (r = - 0.852, P < 0.001) and TNF-α (r = - 0.761, P < 0.001). Overexpression of miR-19b-3p alleviated LPS-induced inflammatory response of HUVECs, which was reflected by the decrease of the levels of IL-6 and TNF-α induced by LPS treatment (P < 0.001).

Conclusion: MiR-19b-3p might be a potential biomarker for the early diagnosis and prognosis of sepsis patients. Overexpression of miR-19b-3p alleviated sepsis-induced inflammatory responses.

Citing Articles

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Zheng X, Zhang Y, Lin S, Li Y, Hua Y, Zhou K PLoS One. 2023; 18(2):e0279726.

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Expression of MicroRNAs in Sepsis-Related Organ Dysfunction: A Systematic Review.

Maiese A, Scatena A, Costantino A, Chiti E, Occhipinti C, La Russa R Int J Mol Sci. 2022; 23(16).

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