Characteristics of the Urinary Microbiome in Kidney Stone Patients with Hypertension

Overview

Journal J Transl Med

Publisher Biomed Central

Specialties Biomedical Engineering
General Medicine

Date 2020 Mar 19

PMID 32183836

Citations 24

Authors

Fengping Liu

Nan Zhang

Peng Jiang

Qixiao Zhai

Chen Li

Deshui Yu

Yan Wu

Yuwei Zhang

Longxian Lv

Xinyu Xu

Ninghan Feng

Affiliations

Soon will be listed here.

Abstract

Background: Kidney stone disease (KSD) is more common in individuals with hypertension (HTN) than in individuals with normotension (NTN). Urinary dysbiosis is associated with urinary tract disease and systemic diseases. However, the role of the urinary microbiome in KSD complicated with HTN remains unclear.

Methods: This study investigated the relationship between the pelvis urinary microbiome and blood pressure (BP) in patients with KSD co-occurring with HTN (KSD-HTN) and healthy controls (HC) by conducting 16S rRNA gene sequencing of bacteria in urine samples. The urine samples were collected (after bladder disinfection) from 50 patients with unilateral kidney calcium stones and NTN (n = 12), prehypertension (pHTN; n = 11), or HTN (n = 27), along with 12 HCs.

Results: Principal coordinates analysis showed that there were significant differences in the urinary microbiomes not only between KSD patients and HCs but also between KSD-pHTN or KSD-HTN patients and KSD-NTN patients. Gardnerella dominated in HCs, Staphylococcus dominated in KSD-NTN patients and Sphingomonas dominated in both KSD-pHTN and KSD-HTN patients. The abundance of several genera including Acidovorax, Gardnerella and Lactobacillus was correlated with BP. Adherens junction and nitrogen and nucleotide metabolism pathways, among others, were associated with changes in BP.

Conclusions: The findings suggest that patients with KSD complicated with HTN have a unique urinary microbiome profile and that changes in the microbiome may reflect disease progression and may be useful to monitor response to treatments.

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References

Villa-Etchegoyen C, Lombarte M, Matamoros N, Belizan J, Cormick G . Mechanisms Involved in the Relationship between Low Calcium Intake and High Blood Pressure. Nutrients. 2019; 11(5). PMC: 6566648. DOI: 10.3390/nu11051112. View

Nienhouse V, Gao X, Dong Q, Nelson D, Toh E, McKinley K . Interplay between bladder microbiota and urinary antimicrobial peptides: mechanisms for human urinary tract infection risk and symptom severity. PLoS One. 2014; 9(12):e114185. PMC: 4259481. DOI: 10.1371/journal.pone.0114185. View

Karstens L, Asquith M, Caruso V, Rosenbaum J, Fair D, Braun J . Community profiling of the urinary microbiota: considerations for low-biomass samples. Nat Rev Urol. 2018; 15(12):735-749. PMC: 6352978. DOI: 10.1038/s41585-018-0104-z. View

Gottschick C, Deng Z, Vital M, Masur C, Abels C, Pieper D . The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome. 2017; 5(1):99. PMC: 5554977. DOI: 10.1186/s40168-017-0305-3. View

Booth 3rd J, Li J, Zhang L, Chen L, Muntner P, Egan B . Trends in Prehypertension and Hypertension Risk Factors in US Adults: 1999-2012. Hypertension. 2017; 70(2):275-284. PMC: 5594566. DOI: 10.1161/HYPERTENSIONAHA.116.09004. View

Almuzara M, Cittadini R, Ocampo C, Bakai R, Traglia G, Ramirez M . Intra-abdominal infections due to Comamonas kerstersii. J Clin Microbiol. 2013; 51(6):1998-2000. PMC: 3716083. DOI: 10.1128/JCM.00659-13. View

Coe F, Kavalach A . Hypercalciuria and hyperuricosuria in patients with calcium nephrolithiasis. N Engl J Med. 1974; 291(25):1344-50. DOI: 10.1056/NEJM197412192912510. View

Mitchell T, Kumar P, Reddy T, Wood K, Knight J, Assimos D . Dietary oxalate and kidney stone formation. Am J Physiol Renal Physiol. 2018; 316(3):F409-F413. PMC: 6459305. DOI: 10.1152/ajprenal.00373.2018. View

Bautista L, Vera L, Arenas I, Gamarra G . Independent association between inflammatory markers (C-reactive protein, interleukin-6, and TNF-alpha) and essential hypertension. J Hum Hypertens. 2004; 19(2):149-54. DOI: 10.1038/sj.jhh.1001785. View

10.

Kittanamongkolchai W, Mara K, Mehta R, Vaughan L, Denic A, Knoedler J . Risk of Hypertension among First-Time Symptomatic Kidney Stone Formers. Clin J Am Soc Nephrol. 2017; 12(3):476-482. PMC: 5338710. DOI: 10.2215/CJN.06600616. View

11.

Yang T, Santisteban M, Rodriguez V, Li E, Ahmari N, Carvajal J . Gut dysbiosis is linked to hypertension. Hypertension. 2015; 65(6):1331-40. PMC: 4433416. DOI: 10.1161/HYPERTENSIONAHA.115.05315. View

12.

Wolfe A, Toh E, Shibata N, Rong R, Kenton K, Fitzgerald M . Evidence of uncultivated bacteria in the adult female bladder. J Clin Microbiol. 2012; 50(4):1376-83. PMC: 3318548. DOI: 10.1128/JCM.05852-11. View

13.

Shankar A, Klein R, Klein B, Nieto F . The association between serum uric acid level and long-term incidence of hypertension: Population-based cohort study. J Hum Hypertens. 2006; 20(12):937-45. DOI: 10.1038/sj.jhh.1002095. View

14.

Li J, Zhao F, Wang Y, Chen J, Tao J, Tian G . Gut microbiota dysbiosis contributes to the development of hypertension. Microbiome. 2017; 5(1):14. PMC: 5286796. DOI: 10.1186/s40168-016-0222-x. View

15.

Zhang W, Sun K, Yang Y, Zhang H, Hu F, Hui R . Plasma uric acid and hypertension in a Chinese community: prospective study and metaanalysis. Clin Chem. 2009; 55(11):2026-34. DOI: 10.1373/clinchem.2009.124891. View

16.

Malani A, Aronoff D, Bradley S, Kauffman C . Cardiobacterium hominis endocarditis: Two cases and a review of the literature. Eur J Clin Microbiol Infect Dis. 2006; 25(9):587-95. PMC: 2276845. DOI: 10.1007/s10096-006-0189-9. View

17.

Fouts D, Pieper R, Szpakowski S, Pohl H, Knoblach S, Suh M . Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury. J Transl Med. 2012; 10:174. PMC: 3511201. DOI: 10.1186/1479-5876-10-174. View

18.

Liu F, Ling Z, Xiao Y, Lv L, Yang Q, Wang B . Dysbiosis of urinary microbiota is positively correlated with type 2 diabetes mellitus. Oncotarget. 2016; 8(3):3798-3810. PMC: 5354796. DOI: 10.18632/oncotarget.14028. View

19.

Sesso H, Buring J, Rifai N, J Blake G, Gaziano J, Ridker P . C-reactive protein and the risk of developing hypertension. JAMA. 2003; 290(22):2945-51. DOI: 10.1001/jama.290.22.2945. View

20.

Mushtaq N, Hussain S, Zhang S, Yuan L, Li H, Ullah S . Molecular characterization of alterations in the intestinal microbiota of patients with grade 3 hypertension. Int J Mol Med. 2019; 44(2):513-522. PMC: 6605625. DOI: 10.3892/ijmm.2019.4235. View