Polyvinyl Alcohol/Chitosan/Polyhexamethylene Biguanide Phase Separation System: A Potential Topical Antibacterial Formulation with Enhanced Antimicrobial Effect

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Mar 19

PMID 32183411

Citations 4

Authors

Yunzhou Ni

Zhixiang Qian

Yu Yin

Weien Yuan

Fei Wu

Tuo Jin

Affiliations

Soon will be listed here.

Abstract

An aqueous polyvinyl alcohol (PVA)/chitosan (CHT)/polyhexamethylene biguanide (PHMB) blends (PVA/CHT/PHMB blends) has been developed as a potential low dose topical antibacterial formulation with enhanced antimicrobial effect. The preparation of PVA/CHT/PHMB blends was quite facilely, with just dissolved PVA, CHT, PHMB in water in order. There was the aggregates with 100 nm size around induced by phase separation in the blends and an aqueous two-phase system (ATPS) formed, as non-ionic polymer PVA formed a continuous phase and cationic polymer CHT and PHMB formed dispersed phases. The minimum inhibitory concentration (MIC) of PHMB in the PVA/CHT/PHMB blends was 0.5μg/mL, which was four times lower than the MIC of PHMB individually. A phase separation increased zeta potential mechanism was proposed to explain the enhanced antibacterial activities. In addition, the blends could easily form film on the skin surface with good water vapor permeability and be used as a liquid bandage to accelerate the scratch wound healing process of nude mouse. These findings provide experimental evidence that the PHMB-functionalized blends could be further explored as low-dose topical antibacterial formulations, and the nano-sized phase separation strategy could be used to design novel low-dose topical antibacterial formulations with an enhanced antimicrobial effect.

Citing Articles

Sterilized Polyhexanide-Releasing Chitosan Membranes with Potential for Use in Antimicrobial Wound Dressings.

Vaz L, Branco R, Morais P, Guiomar A Membranes (Basel). 2023; 13(11).

PMID: 37999363 PMC: 10673555. DOI: 10.3390/membranes13110877.

Antiseptic Chitosan-Poly(hexamethylene) Biguanide Hydrogel for the Treatment of Infectious Wounds.

Antony I, Pradeep A, Pillai A, Menon R, Kumar V, Jayakumar R J Funct Biomater. 2023; 14(10).

PMID: 37888193 PMC: 10607813. DOI: 10.3390/jfb14100528.

Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review.

Guiomar A, Urbano A Membranes (Basel). 2022; 12(12).

PMID: 36557188 PMC: 9781366. DOI: 10.3390/membranes12121281.

Chitosan Hydrochloride Decreases Growth and Virulence and Boosts Growth, Development and Systemic Acquired Resistance in Two Durum Wheat Genotypes.

Francesconi S, Steiner B, Buerstmayr H, Lemmens M, Sulyok M, Balestra G Molecules. 2020; 25(20).

PMID: 33081211 PMC: 7587526. DOI: 10.3390/molecules25204752.

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