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Structural Features of Three Hetero-Galacturonans from Fruits and Their in Vitro Immunomodulatory Effects

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Publisher MDPI
Date 2020 Mar 19
PMID 32182663
Citations 4
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Abstract

is a horticultural plant and vital traditional Chinese herbal medicine. In our previous study, the characterization and immuno-enhancing effect of fruits polysaccharide 1 (PFP1), a water-eluted hetero-mannan from wild fruits, were investigated. Herein, another three salt-eluted novel polysaccharides, namely PFP2, PFP3, and PFP4, were obtained and structurally characterized. The results showed that PFP2, PFP3, and PFP4 were three structurally similar hetero-galacturonans with different molecular weights of 6.11 × 10, 4.37 × 10 and 3.48 × 10 g/mol, respectively. All three of these hetero-galacturonans are mainly composed of galacturonic acid, galactose, arabinose (75.69%, 80.39%, and 74.30%, respectively), and other monosaccharides including mannose, fucose, glucose, ribose, xylose, and glucuronic acid (24.31%, 19.61, and 25.70%, respectively), although differences in their backbone structure exist. Additionally, immunomodulatory assay indicated that the three hetero-galacturonans possess the ability to promote the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in RAW264.7 macrophages in a concentration-dependent manner ( < 0.05). Especially, PFP3 displayed a stronger enhancing effect than PFP2 and PFP4 at the minimum effective concentration. Therefore, the results suggested that the obtained three salt-eluted hetero-galacturonans, especially PFP3, could be utilized as immunomodulatory effectivity ingredients in nutritional/pharmaceutical industries.

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References
1.
Liang X, Gao Y, Pan Y, Zou Y, He M, He C . Purification, chemical characterization and antioxidant activities of polysaccharides isolated from Mycena dendrobii. Carbohydr Polym. 2018; 203:45-51. DOI: 10.1016/j.carbpol.2018.09.046. View

2.
Ahmad N, Chillara R, Kushwaha P, Khedgikar V, Karvande A, Choudhary D . Evaluation of anti-osteoporotic activity of butanolic fraction from Passiflora foetida in ovariectomy-induced bone loss in mice. Biomed Pharmacother. 2017; 88:804-813. DOI: 10.1016/j.biopha.2017.01.100. View

3.
Jiao Y, Hua D, Huang D, Zhang Q, Yan C . Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes. Food Funct. 2018; 9(7):3997-4007. DOI: 10.1039/c8fo00790j. View

4.
Song Y, Wei X, Li M, Duan X, Sun Y, Yang R . Nutritional Composition and Antioxidant Properties of the Fruits of a Chinese Wild Passiflora foetida. Molecules. 2018; 23(2). PMC: 6017921. DOI: 10.3390/molecules23020459. View

5.
Wang L, Zhang B, Xiao J, Huang Q, Li C, Fu X . Physicochemical, functional, and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit. Food Chem. 2018; 249:127-135. DOI: 10.1016/j.foodchem.2018.01.011. View