A Rapid Method for the Functional Reconstitution of Amino Acid Transport Systems from Rat Liver Plasma Membranes. Partial Purification of System A

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Nov 1

PMID 3214433

Citations 13

Authors

A R Quesada

J D McGivan

Affiliations

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Abstract

A rapid method for the functional reconstruction of amino acid transport from liver plasma-membrane vesicles using the neutral detergent decanoyl-N-glucamide ('MEGA-10') is described. The method is a modification of that previously employed in this laboratory for reconstitution of amino acid transport systems from kidney brush-border membranes [Lynch & McGivan (1987) Biochem. J. 244, 503-508]. The transport activities termed 'System A', 'System N', and 'System L' are all reconstituted. The reconstitution procedure is rapid and efficient and is suitable as an assay for transport activity in studies involving membrane fractionation. By using this reconstitution procedure, System A transport activity was partially purified by lectin-affinity chromatography.

Citing Articles

Amino acid transport system A resembles system N in sequence but differs in mechanism.

Reimer R, Chaudhry F, Gray A, EDWARDS R Proc Natl Acad Sci U S A. 2000; 97(14):7715-20.

PMID: 10859363 PMC: 16610. DOI: 10.1073/pnas.140152797.

Reconstitution studies of amino acid transport system L in rat erythrocytes.

Yao S, George R, Young J Biochem J. 1993; 292 ( Pt 3):655-60.

PMID: 8317996 PMC: 1134163. DOI: 10.1042/bj2920655.

Regulatory and molecular aspects of mammalian amino acid transport.

McGivan J, Pastor-Anglada M Biochem J. 1994; 299 ( Pt 2):321-34.

PMID: 8172590 PMC: 1138275. DOI: 10.1042/bj2990321.

Evidence for a regulatory protein involved in the increased activity of system A for neutral amino acid transport in osmotically stressed mammalian cells.

Gomez-Angelats M, Casado F, Felipe A, McGivan J, Pastor-Anglada M Proc Natl Acad Sci U S A. 1994; 91(20):9569-73.

PMID: 7937807 PMC: 44854. DOI: 10.1073/pnas.91.20.9569.

Expression of rat liver Na+/L-alanine co-transport in Xenopus laevis oocytes. Effect of glucagon in vivo.

Palacin M, Werner A, Dittmer J, Murer H, Biber J Biochem J. 1990; 270(1):189-95.

PMID: 2396979 PMC: 1131697. DOI: 10.1042/bj2700189.

References

Dawson R . ON THE MECHANISM OF ACTION OF PHOSPHOLIPASE A. Biochem J. 1963; 88:414-23. PMC: 1202194. DOI: 10.1042/bj0880414. View

POOLE R, Halestrap A . Reconstitution of the L-lactate carrier from rat and rabbit erythrocyte plasma membranes. Biochem J. 1988; 254(2):385-90. PMC: 1135089. DOI: 10.1042/bj2540385. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

van Amelsvoort J, Sips H, van Dam K . Sodium-dependent alanine transport in plasma-membrane vesicles from rat liver. Biochem J. 1978; 174(3):1083-6. PMC: 1186018. DOI: 10.1042/bj1741083. View

Will P, Hopfer U . Apparent inhibition of active non-electrolyte transport by an increased sodium permeability of the plasma membrane. Mechanism of action of p-chloromercuribenzene sulfonate. J Biol Chem. 1979; 254(10):3806-11. View

Biber J, Hauser H . The role of SH-groups in the concentrative transport of D-glucose into brush border membrane vesicles. FEBS Lett. 1979; 108(2):451-6. DOI: 10.1016/0014-5793(79)80586-4. View

Kilberg M, Handlogten M, CHRISTENSEN H . Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs. J Biol Chem. 1980; 255(9):4011-9. View

Sips H, van Amelsvoort J, van Dam K . Amino acid transport in plasma-membrane vesicles from rat liver. Characterization of L-alanine transport. Eur J Biochem. 1980; 105(2):217-24. DOI: 10.1111/j.1432-1033.1980.tb04492.x. View

Sips H, Apitule M, van Dam K . Amino acid transport in plasma membrane vesicles from isolated rat liver parenchymal cells. Biochim Biophys Acta. 1980; 600(2):577-80. DOI: 10.1016/0005-2736(80)90458-7. View

10.

Sips H, van Dam K . Amino acid-dependent sodium transport in plasma membrane vesicles from rat liver. J Membr Biol. 1981; 62(3):231-7. DOI: 10.1007/BF01998168. View

11.

Samson M, Fehlmann M . Plasma membrane vesicles from isolated hepatocytes retain the increase of amino acid transport induced by dibutyryl cyclic AMP in intact cells. Biochim Biophys Acta. 1982; 687(1):35-41. DOI: 10.1016/0005-2736(82)90167-5. View

12.

Kilberg M . Amino acid transport in isolated rat hepatocytes. J Membr Biol. 1982; 69(1):1-12. DOI: 10.1007/BF01871236. View

13.

Hildreth J . N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem J. 1982; 207(2):363-6. PMC: 1153869. DOI: 10.1042/bj2070363. View

14.

Shotwell M, Kilberg M, OXENDER D . The regulation of neutral amino acid transport in mammalian cells. Biochim Biophys Acta. 1983; 737(2):267-84. DOI: 10.1016/0304-4157(83)90003-5. View

15.

Hayes M, McGivan J . Comparison of the effects of certain thiol reagents on alanine transport in plasma membrane vesicles from rat liver and their use in identifying the alanine carrier. Biochem J. 1983; 214(2):489-95. PMC: 1152271. DOI: 10.1042/bj2140489. View

16.

Barber E, Handlogten M, Kilberg M . Induction of amino acid transport system A in rat hepatocytes is blocked by tunicamycin. J Biol Chem. 1983; 258(19):11851-5. View

17.

Koepsell H, Korn K, Ferguson D, Menuhr H, Ollig D, Haase W . Reconstitution and partial purification of several Na+ cotransport systems from renal brush-border membranes. Properties of the L-glutamate transporter in proteoliposomes. J Biol Chem. 1984; 259(10):6548-58. View

18.

McCormick J, Tsang D, Johnstone R . A simple and efficient method for reconstitution of amino acid and glucose transport systems from Ehrlich ascites cells. Arch Biochem Biophys. 1984; 231(2):355-65. DOI: 10.1016/0003-9861(84)90398-9. View

19.

Kilberg M, Barber E, Handlogten M . Characteristics and hormonal regulation of amino acid transport system A in isolated rat hepatocytes. Curr Top Cell Regul. 1985; 25:133-63. DOI: 10.1016/b978-0-12-152825-6.50009-6. View

20.

Bracy D, Schenerman M, Kilberg M . Solubilization and reconstitution of hepatic System A-mediated amino acid transport. Preparation of proteoliposomes containing glucagon-stimulated transport activity. Biochim Biophys Acta. 1987; 899(1):51-8. DOI: 10.1016/0005-2736(87)90238-0. View